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Toxicologic Pathology
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Journal Article

Immunohistochemical and Biochemical Identification of Pepsinogen Isozymes in the Hamster Lungs: Induction by Polychlorinated Biphenyls

Katsumi Imaida

Division of Pathology, National Institute of Hygienic Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158, Japan

Chie Furihata

Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan

Masae Tatematsu

Department of Pathology, Nagoya City University Medical School, Mizuho-cho, Mizuho-ku, Nagoya 467 Japan

Cheol Ho Yoon

Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, Japan

Fumio Furukawa

Division of Pathology, National Institute of Hygienic Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158, Japan

Chikako Uneyama

Division of Pathology, National Institute of Hygienic Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158, Japan

Michihito Takahashi

Division of Pathology, National Institute of Hygienic Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158, Japan

Nobuyuki Ito

Department of Pathology, Nagoya City University Medical School, Mizuho-cho, Mizuho-ku, Nagoya 467 Japan

Yuzo Hayashi

Division of Pathology, National Institute of Hygienic Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158, Japan

Pesinogens are acid protease enzymes of pepsin usually found in gastric mucosa. In the present study, we demonstrated the presence of pepsinogen isozymes in male Syrian golden hamster lung tissues by a combined immunohistochemical and biochemical approach. Immunohistochemically, using rat pepsinogen 1 antibody, pepsinogen positive cells were observed mainly in the epithelia of the terminal bronchioles. They demonstrated morphological features of Clara cells. The pepsinogen isozyme pattern of lung tissue determined by polyacrylamide gel electrophoresis was similar to that of stomach mucosa. Treatment of hamsters with polychlorinated biphenyls at a dose of 500 mg/kg body weight ip caused a 2.8-fold increase in pepsinogen content (p < 0.01) as well as increase in numbers of pepsinogen positive cells in the lung.

Key Words: Hamster • lung • pepsinogen • polychlorinated biphenyls • Clara cells • immunohistochemistry • enzyme

Toxicologic Pathology, Vol. 19, No. 3, 230-236 (1991)
DOI: 10.1177/019262339101900305
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