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c-N-ras is Activated Infrequently in Canine Malignant LymphomaDepartment of Surgery, School of Veterinary Medicine, University of California, Davis. California 95616–8745
Department of Surgery, School of Veterinary Medicine, University of California, Davis. California 95616–8745
Cancer and Molecular Research Laboratory, UC Davis Cancer Center, Sacramento, California 95817
Department of Surgery, School of Veterinary Medicine, University of California, Davis. California 95616–8745 Activated c-N-ras alleles have been detected in human lymphoma specimens. The aim of the present study was to determine the frequency of c-N-ras mutational activation in canine malignant lymphoma. DNA was isolated from 28 canine malignant lymphoma specimens collected from 28 separate dogs and examined for c-N-ras mutations by polymerase chain reaction amplification and direct sequencing. The tumors were naturally occurring and derived from 20 dogs with known exposures to the phenoxy herbicide 2,4-dichlo-rophenoxyacetic acid (2,4-D) and from 8 dogs with no known exposure to the herbicide. An oncogenically activating mutation was found in 1 dog without known 2,4-D exposure. The mutation was a 13th codon, second position transition that would result in a glycine-to-aspartate amino acid substitution. The results of this study demonstrate that, similar to the human, c-N-ras mutations are uncommon in dogs with malignant lymphoma and that there is no association between 2,4-D exposure and activation of c-N-ras in the dog.
Key Words: ras oncogenes • 2,4-dichlorophenoxyacetic acid • polymerase chain reaction
Toxicologic Pathology, Vol. 21, No. 3,
288-291 (1993) |
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