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Toxicological Evaluation of u-hEGFIstituto di Ricerche Biomediche "Antoine Marxer," RBM S.p.A. Ivrea (Torino), Italy
Istituto di Ricerche Biomediche "Antoine Marxer," RBM S.p.A. Ivrea (Torino), Italy
Istituto di Ricerche Biomediche "Antoine Marxer," RBM S.p.A. Ivrea (Torino), Italy
Istituto di Ricerche Biomediche "Antoine Marxer," RBM S.p.A. Ivrea (Torino), Italy
Istituto di Ricerche Biomediche "Antoine Marxer," RBM S.p.A. Ivrea (Torino), Italy
Istituto di Ricerche Biomediche "Antoine Marxer," RBM S.p.A. Ivrea (Torino), Italy, Koret School of Veterinary Medicine, University of Jerusalem, Israel The toxicological evaluation of urinary human epidermal growth factor (u-hEGF) included mutagenicity, single and repeated dose general toxicity, and teratogenicity studies in various animal species. The mutagenic potential of u-hEGF was tested in vitro (Ames test, chromosome aberration in human lymphocytes, unscheduled DNA synthesis in HeLa cells) and in vivo (chromosome aberration in Chinese hamster bone marrow and micronucleus test in rat bone marrow). No mutagenic or clastogenic effects were found. The acute toxicity of u-hEGF was evaluated in mice and rats, using single subcutaneous (sc) or intravenous (iv) injection of 15 mg/kg. No toxic effects were observed. Four-week iv daily administration of u-hEGF at the doses of 0.3, 0.9, and 3 mg/kg in the SD rat followed by 2 wk of compound withdrawal induced pronounced and generally dose-related effects (i.e., epithelial hyperplasia) in a wide range of tissues and organs, at all doses. However, these effects were not apparently detrimental to the general health of the rats. The repeated sc administration of u-hEGF to cynomolgus monkeys for 4 wk at the same doses as used in the rat study resulted in lethality after about 7 days of treatment in the 2 higher dose groups or after 14 days at the lowest dose. The main clinical signs observed were gastrointestinal effects, respiratory distress, sedation, marked loss of body weight, and cutaneous desquamation. At histology, hyperplasia of most epithelia was seen in all groups. In addition, atrophy of the ovarian follicles and necrosis of the uterine endometrium were noted. Changes considered secondary to physical distress were atrophy of the hemopoietic and lymphatic system and hepatic steatosis. The embryofetal toxicity and teratogenicity of u-hEGF was tested, using the iv route in the SD rat and the iv and sc routes in the New Zealand White rabbit. In both species, the compound was administered at the doses of 0, 0.3, 0.9, and 3 mg/kg/day, from day 6 to 15 of pregnancy in rats and 6-18 in rabbits. In the rat, an increase in body weight was noted in the dams and fetuses at the 2 high doses. No embryotoxic or teratogenic effects were observed. In the rabbit studies, mortality and severe clinical signs involving various systems, with marked effects on the eyes, were observed at all doses tested during the first days of treatment by both routes. From the reproductive point of view, most of the surviving treated gravid females showed only resorptions.
Key Words: Urinary human epidermal growth factor • epithelial hyperplasia • rat • monkey • rabbit
Toxicologic Pathology, Vol. 23, No. 3,
356-366 (1995) This article has been cited by other articles:
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