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Toxicologic Pathology
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P53 Accumulation in N-Methyl-N-Nitrosourea-Induced Rat Mammary Tumors

Keith A. Crist

Department of Surgery, Medical College of Ohio

Raymond D. Fuller

Department of Surgery, Medical College of Ohio

Bina Chaudhuri

Department of Pathology, Medical College of Ohio, 3000 Arlington Avenue, Toledo, Ohio 43699-0008

Prabir Chaudhuri

Department of Surgery, Medical College of Ohio

Ming You

Department of Pathology, Medical College of Ohio, 3000 Arlington Avenue, Toledo, Ohio 43699-0008

P53 aberrant protein expression and mutation is a component of many human tumors but less information is available regarding involvement in relevant animal models. We have examined invasive mammary epithelial neoplasms for p53 aberrant protein expression from Sprague-Dawley rats induced by two intrajugular injections of N-methyl-N-nitrosourea (NMU, 50 mg/kg), 7 days apart, beginning at 44-49 days of age. Positive nuclear staining was observed in 8/10 mammary tumor frozen sections using PAb 421, a mouse monoclonal antibody, compared to a mixed mouse IgG negative control antibody. Paraffin sections from two additional sets of similarly induced rat mammary tumors also showed positive nuclear staining (22/37 tumors) with CM-5, a rabbit polyclonal antibody. Our results indicate that elevated cellular content of p53 is a common event in invasive palpable mammary tumors induced by NMU in this dual-injection model system.

Key Words: Immunohistochemistry • breast • adenocarcinoma • estrus • peroxidase • female

Toxicologic Pathology, Vol. 24, No. 3, 370-375 (1996)
DOI: 10.1177/019262339602400314
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