This Article Free Full Text (Free PDF) Free References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Whittaker, P. Articles by Dunkel, V. C. Search for Related Content PubMed PubMed Citation Articles by Whittaker, P. Articles by Dunkel, V. C. Social Bookmarking                         What's this?

Histopathological Evaluation of Liver, Pancreas, Spleen, and Heart from Iron-Overloaded Sprague-Dawley Rats*^1,2

Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D.C. 20204

Fred A. Hines

Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D.C. 20204

Martin G. Robl

Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D.C. 20204

Virginia C. Dunkel

Center for Food Safety and Applied Nutrition, Food and Drug Administration, Washington, D.C. 20204

The effects of increasing dietary levels of Fe on the histopathology of liver, pancreas, spleen, and heart were examined in a rat model for iron overload. Sprague-Dawley rats were fed diets containing 35, 350, 3,500, or 20,000 µg Fe/g, and, after 12 wk, there was a direct correlation between increased liver nonheme Fe and lipid peroxidation measured by the lipid-conjugated diene assay. Histopathological examination of tissues revealed the following: (a) hepatocellular hemosiderosis in all groups of rats, with a dose-related accumulation of cytoplasmic Fe-positive material predominantly in hepatocytes located in the periportal region (Zone 1), (b) myocardial degeneration and necrosis (cardiomyopathy) with hemosiderin in interstitial macrophages or in myocardial fibers of animals with heart damage, (c) splenic lymphoid atrophy affecting the marginal zone of the white pulp and hemosiderin deposition in the sinusoidal macrophages, and (d) pancreatic atrophy with loss of both the endocrine and exocrine pancreatic tissue in those animals receiving 3,500 and 20,000 µg Fe/g of diet. The toxic effects of Fe overload in this rat model include cellular apoptosis or necrosis in heart, spleen, and pancreas and, when coupled with the findings on lipid peroxidation, suggests that oxidative stress is involved in the pathogenesis of the lesions.

Key Words: Iron toxicity • hepatocellular hemosiderosis • pancreatic atrophy • cardiotoxicity • splenic atrophy • apoptosis

Toxicologic Pathology, Vol. 24, No. 5, 558-563 (1996)
DOI: 10.1177/019262339602400504


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. H. Kramer, S. B. Murthi, R. M. Wise, I-T. Mak, and W. B. Weglicki Antioxidant and lysosomotropic properties of acute d-propranolol underlies its cardioprotection of postischemic hearts from moderate iron-overloaded rats. Experimental Biology and Medicine, April 1, 2006; 231(4): 473 - 484. [Abstract] [Full Text] [PDF]

				C. K. Yeung, L. Zhu, R. P. Glahn, and D. D. Miller Iron Absorption from NaFeEDTA Is Downregulated in Iron-Loaded Rats J. Nutr., September 1, 2004; 134(9): 2270 - 2274. [Abstract] [Full Text] [PDF]

				I. Narama, K. Ozaki, S. Matsushima, and T. Matsuura Eosinophilic Gastroenterocolitis in Iron Lactate-Overloaded Rats Toxicol Pathol, May 1, 1999; 27(3): 318 - 324. [Abstract] [PDF]

				M. A. Breider, H. M. Ulloa, D. G. Pegg, and A. W. Gough Nitro-Imidazole Radiosensitizer-Induced Toxicity in Cynomolgus Monkeys Toxicol Pathol, September 1, 1998; 26(5): 651 - 656. [Abstract] [PDF]

				P. Whittaker, V. C. Dunkel, T. J. Bucci, D. F Kusewitt, J. D. Thurman, A. Warbritton, and G. L. Wolff Genome-Linked Toxic Responses to Dietary Iron Overload Toxicol Pathol, November 1, 1997; 25(6): 556 - 564. [Abstract] [PDF]