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Toxicologic Pathology
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The Effect of Erythritol on the Stability of {gamma}-glutamyl Transpeptidase and N-acetyl Glucosaminidase in Human Urine

Walter F. Loeb

Ani Lytics Inc., Suite 200, 200 Girard Street, Gaithersburg, Maryland 20877

Saroj R. Das

Ani Lytics Inc., Suite 200, 200 Girard Street, Gaithersburg, Maryland 20877

J. Richard Trout

Statistics Department, Rutgers University, New Brunswick, New Jersey 08903

In toxicology studies and clinical trials of erythritol, treated animals and human subjects had higher urine {gamma}-glutamyl transpeptidase [{gamma}-glutamyl transferase ({gamma}-GT)] than untreated controls. It has previously been reported that {gamma}-GT activity in frozen urine decreases with time; therefore, a study was undertaken to examine the effects of storage temperature, time, and the presence of erythritol on the stability of {gamma}-GT and N-acetyl glucosaminidase in human urine. In this study, it was found that the rate of decrease of the activity of {gamma}-GT is much greater at -20°C than at -70°C. Variation in the storage temperature of the frozen urine is particularly deleterious to {gamma}-GT. The addition of erythritol in a concentration of 5% reduces this decrease. Approximately 15% of N-acetyl glucosaminidase activity is lost in the initial process of freezing the urine. Thereafter, conditions of temperature, time, and the presence or absence of erythritol account for little additional loss of activity.

Key Words: Enzyme stability • urine storage conditions • erithritol-enzyme interactions

Toxicologic Pathology, Vol. 25, No. 3, 264-267 (1997)
DOI: 10.1177/019262339702500303
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W. F. Loeb
The Measurement of Renal Injury
Toxicol Pathol, January 1, 1998; 26(1): 26 - 28.
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