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Revolution through Genomics in Investigative and Discovery Toxicology

Genomic Sequencing Center, Monsanto Life Sciences Company, St. Louis, Missouri 63198, charles.p.rodi @ monsanto.com.

Roderick T. Bunch

Monsanto Safety Evaluation, G.D. Searle/Monsanto, Skokie, Illinois 60077

Sandra W. Curtiss

Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167

Larry D. Kier

Nutrition Sector, Monsanto Life Sciences Company, St. Louis, Missouri 63167

Marc A. Cabonce

Nutrition Sector, Monsanto Life Sciences Company, St. Louis, Missouri 63167

Julio C. Davila

Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167

Michael D. Mitchell

Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167

Carl L. Alden

Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167

Dale L. Morris

Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167

The remarkable technologic and methodologic advances spurred on by the Human Genome Project are being applied throughout the life sciences. In the field of toxicology, high-resolution assays now make it possible to discover virtually all the differences in gene expression brought on by exposure to a particular xenobiotic. There are 2 principal approaches used to build a catalog of changes in gene expression: hybridization microarrays and gel-based methods, such as differential display and AFLP^TM-based mRNA fingerprinting. The power of such approaches is exemplified by the identification of more than 300 genes that differ in expression level by at least 2-fold in response to the nongenotoxic rodent liver carcinogen phenobarbital.

Key Words: Genomics • toxicology • gene expression • phenobarbital • liver carcinogen

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