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Toxicologic Pathology
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Quantitative Analysis of the Lobular Distribution of S-Phase in Rat Liver Following Dietary Administration of Di(2-ethylhexyl)phthalate

Anthony R. Soames

Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, United Kingdom

Suzanne Cliffe

Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, United Kingdom

Ian Pate

Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, United Kingdom

John R. Foster

Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, United Kingdom

A simple image-analysis method is described, whereby the distribution of hepatocytes that have entered S-phase, as distinguished by the incorporation of bromodeoxyuridine, can be related to the position of the central and portal veins of the hepatic lobule. Hepatocyte S-phase was induced in the livers of male and female F344 rats by administration of di(2-ethylhexyl)phthalate (DEHP) in the diet for 7 days at 2 dose levels, and these livers were used to develop the procedure. The distributions of the S-phase between control and DEHP-treated livers were compared using statistical techniques. The results showed notable differences in the distribution of S-phase between male and female rats as well as limited dose-related effects.

Key Words: Carcinogenic prediction • di(2-ethylhexyl)phthalate • hepatocyte S-phase • quantitative lobular distribution • rat

Toxicologic Pathology, Vol. 27, No. 4, 436-440 (1999)
DOI: 10.1177/019262339902700407
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