Toxicologic Pathology

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Register here to gain access to SAGE's 500+ Journals Online

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Free Full Text (Free PDF) Free
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Konishi, N.
Right arrow Articles by Emi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Konishi, N.
Right arrow Articles by Emi, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Toxicologic Pathology, Vol. 29, No. 2, 232-236 (2001)
DOI: 10.1080/019262301317052503

Specific Genomic Alterations in Rat Renal Cell Carcinomas Induced by N-Ethyl-N-hydroxyethylnitrosamine

Noboru Konishi

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, nkonishi{at}naramed-u.ac.jp

Mitsutoshi Nakamura

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521

Eiwa Ishida

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521

Yoichi Kawada

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521

Masayoshi Nishimine

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521

Hisaki Nagai

Department of Molecular Biology, Institute of Gerontology, Nippon Medical School 1-396, Kosugi, Nakahara-ku, Kawasaki, 211-8533, Japan

Mitsuru Emi

Department of Molecular Biology, Institute of Gerontology, Nippon Medical School 1-396, Kosugi, Nakahara-ku, Kawasaki, 211-8533, Japan

To characterize genetic alterations occurring in renal tumorigenesis, EHEN-induced renal cell tumors were examined using restriction landmark genomic scanning (RLGS) analysis, an electrophoretic separation technique that detects gene amplifi cations and deletions. Comparison of DNAs from tumor against those from corresponding nontumorous kidney and /or EHEN-treated kidney without development of renal tumors yielded specific alterations in terms of both amplifi ed and reduced DNA spots. Two amplifi ed spots were detected only in renal cell tumors and an additional four spots were frequent in EHEN-treated kidneys. One reduced spot was common to all tumor samples, and another was frequently detected in the tumors analyzed but not in EHEN-treated kidneys. A subset of the altered spots thus appeared to be specific for EHEN-induced renal tumorigenesis.

Key Words: N-ethyl-N-hydroxyethylnitrosamine • rat • renal cell tumor • restriction landmark genomic scanning.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?