Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to register for free online access

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Toxicologic Pathology
This Article
Right arrow Free Full Text Free
Right arrow Free Full Text (Free PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Muskhelishvili, L.
Right arrow Articles by Latendresse, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Muskhelishvili, L.
Right arrow Articles by Latendresse, J. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Articles

Proliferating Cell Nuclear Antigen—A Marker for Ovarian Follicle Counts

Levan Muskhelishvili, Susan K. Wingard and John R. Latendresse

Toxicologic Pathology Associates at National Center for Toxicological Research, Jefferson, Arkansas 72079, USA

Correspondence: Address correspondence to: Dr. Levan Muskhelishvili, Toxicologic Pathology Associates at National Center for Toxicological Research, 3900 NCTR Road, MC 923, Jefferson, Arkansas 72079, USA; e-mail:lmuskhelishvili{at}nctr.fda.gov

Enumerating ovarian follicles is an effective way to estimate the extent of ovarian toxicity in female rodents exposed to xenobiotics. Differential follicle counts are useful in safety assessment bioassays and in interspecies extrapolation of ovarian toxicity. Counting the follicles in H&E-stained sections is labor intensive, tedious, and costly. In the present study we demonstrated that in rat formalin-fixed, paraffin-embedded ovary sections follicles of all degrees of maturity can be visualized by the use of antibody directed against proliferating cell nuclear antigen (PCNA). Follicles are easily distinguished from ovarian background with the ability to detect and identify primordial follicles being enhanced. This translates into a significant decrease in variability of follicle counts, labor, and cost. Specifically, variability dropped from 11% to 0.2%, the counting time was reduced by 46%, and the cost by 48%.

Key Words: PCNA • immunohistochemistry • ovarian follicles • counts

Toxicologic Pathology, Vol. 33, No. 3, 365-368 (2005)
DOI: 10.1080/01926230590930164
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Toxicol PatholHome page
C. A. Picut, C. L. Swanson, K. L. Scully, V. C. Roseman, R. F. Parker, and A. K. Remick
Ovarian Follicle Counts Using Proliferating Cell Nuclear Antigen (PCNA) and Semi-Automated Image Analysis in Rats
Toxicol Pathol, July 1, 2008; 36(5): 674 - 679.
[Abstract] [Full Text] [PDF]