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Toxicologic Pathology
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Articles

Development of a Percutaneous Cerebrospinal Fluid Collection Technique in F-344 Rats and Evaluation of Cell Counts and Total Protein Concentrations

Alok K. Sharma1,2, Albert E. Schultze2, Dale M. Cooper2, Rachel Y. Reams2, William H. Jordan2 and Paul W. Snyder1

1 Department of Veterinary Pathobiology, Purdue University, West Lafayette, Indiana 47906, USA
2 Department of Pathology, Lilly Research Laboratories, Division of Eli Lilly and Co., Greenfield, Indiana 46140, USA

Correspondence: Address correspondence to: Alok K. Sharma, Department of Pathology, Lilly Research Laboratories, Division of Eli Lilly and Co., Greenfield, Indiana 46140, USA; e-mail:asharma{at}purclue.edu

A simple technique for cerebrospinal fluid (CSF) collection was developed in F-344 rats. Cell counts and total protein concentrations were evaluated to assess sample quality. While the 50 to 70 µL samples of CSF collected on three different days showed a progressive decrease in the total erythrocyte and nucleated cell counts, no significant changes were observed in the total protein concentrations. Progressive decreases in the total erythrocyte count correlated positively with the decreases in volume of CSF collected. Our data suggest that collection of less than 50 µL of CSF will give a better quality of CSF in F-344 rats. This is the first report of cellular and protein parameters in the CSF of F-344 rats.

Key Words: F-344 rats • cerebrospinal fluid • CSF • erythrocytes • nucleated cells • total protein concentration

Toxicologic Pathology, Vol. 34, No. 4, 393-395 (2006)
DOI: 10.1080/01926230600798609
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