Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Toxicologic Pathology
This Article
Right arrow Free Full Text Free
Right arrow Free Full Text (Free PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Kaewamatawong, T.
Right arrow Articles by Takano, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaewamatawong, T.
Right arrow Articles by Takano, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Articles

Acute and Subacute Pulmonary Toxicity of Low Dose of Ultrafine Colloidal Silica Particles in Mice after Intratracheal Instillation

Theerayuth Kaewamatawong1, Akinori Shimada1, Mina Okajima1, Hiromi Inoue1, Takehito Morita1, Kenichiro Inoue2 and Hirohisa Takano2

1 Department of Veterinary Pathology, Tottori University, Minami 4-101, Koyama, Tottori-shi, Tottori 680-8553, Japan
2 National Institute for Environmental Studies, Onogama 16-2, Tsukuba 305-8506, Japan

Correspondence: Address correspondence to: Akinori Shimada, Department of Veterinary Pathology, Tottori University, Minami 4-101, Koyama, Tottori-shi, Tottori 680-0945, Japan. E-mail:aki{at}muses.tottori-u.ac.jp

To study the acute and subacute lung toxicity of low dose of ultrafine colloidal silica particles (UFCSs), mice were intratracheally instilled with 0, 0.3, 3, 10, 30 or 100 µg of UFCSs. Cellular and biochemical parameters in bronchoalveolar lavage fluid (BALF), histological alteration and the body weight were determined at 3 days after instillation. Exposure to 30 or 100 µg of UFCSs produced moderate to severe pulmonary inflammation and tissue injury. To investigate the time response, mice were instilled with 30 µg of UFCSs and sacrificed at intervals from 1 to 30 days post-exposure. UFCSs induced moderate pulmonary inflammation and injury on BALF indices at acute period; however, these changes gradually regressed until recovery during the experiment. Concomitant histopathological and laminin immunohistochemical findings generally correlated to BALF data. TUNEL analyses in UFCSs-treated animals showed a significant increase of the apoptotic index in lung parenchyma at all observation times. 8-OHdG expression occurred in lung epithelial cells and activated macrophages, which correlated to lung lesions in UFCSs-treated mice. These findings suggest that instillation of a small dose of UFCSs causes transient acute moderate lung inflammation and tissue damage. Oxidative stress and apoptosis may underlie the lung tissue injury induction.

Key Words: Apoptosis • bronchoalveolar lavage • intratracheal instillation • lung toxicity • oxidative stress • ultrafine colloidal silica

Toxicologic Pathology, Vol. 34, No. 7, 958-965 (2006)
DOI: 10.1080/01926230601094552
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?