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A Comprehensive Antibody Panel for Immunohistochemical Analysis of Formalin-Fixed, Paraffin-Embedded Hematopoietic Neoplasms of Mice: Analysis of Mouse Specific and Human Antibodies Cross-Reactive with Murine Tissue

Institute of Pathology, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany

Julia Calzada-Wack

Institute of Pathology, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany

Gabriele Hölzlwimmer

Institute of Pathology, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany

Jacqueline Müller

Institute of Pathology, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany

Claudia Kloss

Institute of Pathology, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany

Will Howat

Wellcome Trust Sanger Institute, Cambridge, CB10 1SA United Kingdom

Jörg Schmidt

Department of Comparative Medicine, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany

Heinz Höfler

Institute of Pathology, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany

Madhuri Warren

Wellcome Trust Sanger Institute, Cambridge, CB10 1SA United Kingdom

Leticia Quintanilla-Martinez

Institute of Pathology, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany, Quintanilla-Fend{at}gsf.de

Immunohistochemistry is an indispensable tool in human pathology enabling immunophenotypic characterization of tumor cells. Immunohistochemical analyses of mouse models of human hematopoietic neoplasias have become an important aspect for comparison of murine entities with their human counterparts. The aim of this study was to establish a diagnostic antibody panel for analysis of murine lymphomas/leukemias, useful in formalin-fixed/paraffin-embedded tissue. Overall, 48 antibodies (4 rabbit monoclonal, 12 rabbit polyclonal, 2 goat polyclonal, 11 rat, and 19 mouse monoclonal), which were either mouse-specific (14) or cross-reactive with murine tissue (34) were tested for staining quality and diagnostic value in 468 murine hematopoietic neoplasms. Specific staining was achieved with 29 antibodies, of which 18 were human antibodies cross-reactive with murine tissue. Only 23 (B220, BCL-2, BCL-6, CD117, CD138 (2x), CD3 (2x), CD43, CD45, CD5, CD79cy, cyclin D1, Ki-67 (2x), Mac-3, Mac-2, lysozyme, mast cell tryptase, MPO, Pax-5, TdT, and TER-119) were regarded as valuable for diagnostic evaluation. Immunohistochemistry was also established in an automated immunostainer for high throughput analysis. The antibody panel developed is useful for the classification of murine lymphomas and leukemias analyzed, and a valuable tool for human and veterinary pathologists involved in the diagnostic interpretation of murine models of hematopoietic neoplasias.

Key Words: Hematopathology • immunohistochemistry • mouse pathology.

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