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Toxicologic Pathology
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Articles

Time-Dependent Changes in Post-Mortem Testis Histopathology in the Rat

Bronwyn H. Bryant and Kim Boekelheide

Department of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island 02912

Correspondence: Address correspondence to: Kim Boekelheide, M.D., Ph.D., Department of Pathology and Laboratory Medicine, 70 Ship Street, Box G-E505, Brown University, Providence, RI 02903, USA; e-mail: KimBoekelheide{at}brown.edu

To clarify the contribution of spontaneous or autolytic post-mortem changes to testis histopathology, the testes of adult rats were examined after animals were left at room temperature for 12, 24, 36, and 48 hours postmortem (n = 2 for all time points except 0 hours postmortem, where n = 3). A progressive decrease in testis weight and seminiferous tubule diameter was observed, as well as detachment of the seminiferous epithelium from the basement membrane. As early as 12 hours postmortem, there was observable clumping and margination of chromatin in Leydig cells, Sertoli cells, spermatogonia, spermatocytes, and step 7–10 spermatids; extensive disintegration of Sertoli cells and residual bodies by 24 hours postmortem; and TUNEL positivity of Leydig cells (by 36 hours postmortem) and step 19 spermatids (at 48 hours postmortem). These findings will aid in ensuring proficient histopathological analysis of testes in toxicity studies.

Key Words: Testis • post-mortem • toxicology • autolysis • rat • histopathology

Abbreviations: TUNEL, Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling • ROS, reactive oxygen species

Toxicologic Pathology, Vol. 35, No. 5, 665-671 (2007)
DOI: 10.1080/01926230701459994
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