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Toxicologic Pathology, Vol. 35, No. 7, 952-957 (2007)
DOI: 10.1080/01926230701748198

Articles

The Demonstration of Immunohistochemical Biomarkers in Methyl Methacrylate-Embedded Plucked Human Hair Follicles

Kevin J Randall and John R. Foster

AstraZeneca, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK

Correspondence: Address correspondence to Kevin J. Randall, AstraZeneca, 23G5 Mereside, Alderley Park, Macclesfield, Cheshire, UK SK10 4TG; e-mail:kevin.randall{at}astrazeneca.com

Plucked human hair follicles have been proposed as a potential surrogate for tumour tissue for measuring the effect of drugs on pharmacodynamic biomarkers in drug intervention studies. We describe a new technique of embedding plucked hair follicles in the acrylic resin, methyl methacrylate, and the immunohistochemical demonstration of six potential biomarkers (Ki67, EGFR, phospho-p27, phospho-histone H3, phospho-MAPK and phospho-Rb) in de-plasticised sections. The advantages of this technique over those that have been used in support of clinical drug trials, such as skin and tumour biopsies, whole blood and whole hair samples is discussed.

Key Words: Hair Follicle • methyl methacrylate • immunohistochemistry • drug development • biomarker • pharmacodynamic

Abbreviations: FFPE, formalin-fixed paraffin embedded • IHC, immunohistochemistry • PD, pharmacodynamic


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