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Toxicologic Pathology
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*STYRENE-7,8-OXIDE
*TRITIUM
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Journal Article

Subcellular Distribution of Styrene Oxide in Rat Liver

Gian Maria Pacifici

Department of General Pathology, Medical School, University of Pisa, Italy, and Department of Clinical Pharmacology, Karolinksa Institute, Huddinge University Hospital, Huddinge, Sweden.

Lulgi Cuoci

Department of General Pathology, Medical School, University of Pisa, Italy, and Department of Clinical Pharmacology, Karolinksa Institute, Huddinge University Hospital, Huddinge, Sweden.

Anders Rane

Department of General Pathology, Medical School, University of Pisa, Italy, and Department of Clinical Pharmacology, Karolinksa Institute, Huddinge University Hospital, Huddinge, Sweden.

The subcellular distribution of (3H)-styrene-7,8-oxide was studied in the rat liver. The compound was added to liver homogenate to give a final concentration of 2 times 10–5; 2 times 10–4 and 2 times 10–3 M. Subcellular fractions were obtained by differential centrifugation. Most of styrene oxide (59-88%) was associated with the cytosolic fraction. Less than 15 percent of the compound was retrieved in each of the nuclear, mitochondrial and microsomal fractions. A considerable percentage of radioactivity was found unextractable with the organic solvents, suggesting that styrene oxide reacted with the endogenous compounds. The intracellular distribution of this epoxide was also studied in the perfused rat liver. Comparable results with those previously described were obtained. The binding of styrene oxide to the cytosolic protein was investigated by equilibrium dialysis and ultrafiltration. Only a small percentage of the compound was bound to protein.

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Toxicologic Pathology, Vol. 12, No. 1, 69-73 (1984)
DOI: 10.1177/019262338401200111
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This Article
Right arrow Abstract Freely available
Right arrow Free Full Text (Free PDF) Free
Right arrow Alert me when this article is cited
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Services
Right arrow Email this article to a friend
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Right arrow Similar articles in PubMed
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Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Pacifici, G. M.
Right arrow Articles by Rane, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pacifici, G. M.
Right arrow Articles by Rane, A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*STYRENE-7,8-OXIDE
*TRITIUM
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?