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Phenotyping Cytochromes P450 with Monoclonal Antibodies
Fred K. Friedman
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205
Sang Shin Park
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205
Tadahiko Fujino
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205
Byung-Joon Song
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205
Richard C. Robinson
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205
Donna West
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205
Allen K. Radkowsky
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205
Haruko Miller
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205
Harry V. Gelboin
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20205
Monoclonal antibodies (MAbs) to cytochrome P-450 isozymes can be used to phenotype tissues for epitope-specific cytochrome P-450 content. MAbs that inhibit specific cytochrome P-450 dependent drug or carcinogen reactions are useful tools for quantitative measurement of the individual or classes of cytochromes P-450 that catalyze these reactions. This method has been applied successfully to animal as well as human tissues. Radioimmunoassays based on MAbs have been developed and provide a rapid and efficient means for detecting cytochromes P-450 independent of functional enzyme activity. In addition, MAbs coupled to a Sepharose support can be used to immunopurify cytochromes P-450 in a procedure that is more rapid and efficient than conventional purification schemes. MAbs add a new dimension to analyses of cytochrome P-450 multiplicity and will find numerous applications in elucidation of the relationship between cytochrome P-450 phenotype and carcinogen or drug metabolism.
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Toxicologic Pathology, Vol. 12, No. 2,
155-161 (1984)
DOI: 10.1177/019262338401200207
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