Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Sign In to gain access to subscriptions and/or personal tools.
Toxicologic Pathology
This Article
Right arrow Abstract Freely available
Right arrow Free Full Text (Free PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Barrera, G.
Right arrow Articles by Dianzani, M. U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barrera, G.
Right arrow Articles by Dianzani, M. U.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Journal Article

Effect of 4-Hydroxynonenal on c-myc Expression

Giuseppina Barrera

Istituto di Patologia Generale, Corso Raffaello 30, Torino, Italy, and Istituto di Patologia Generale, Università "La Sapienza," Viale Regina Elena 324, Roma, Italy

Stefano Martinotti

Istituto di Patologia Generale, Corso Raffaello 30, Torino, Italy, and Istituto di Patologia Generale, Università "La Sapienza," Viale Regina Elena 324, Roma, Italy

Vito Fazio

Istituto di Patologia Generale, Corso Raffaello 30, Torino, Italy, and Istituto di Patologia Generale, Università "La Sapienza," Viale Regina Elena 324, Roma, Italy

Vittorio Manzari

Istituto di Patologia Generale, Corso Raffaello 30, Torino, Italy, and Istituto di Patologia Generale, Università "La Sapienza," Viale Regina Elena 324, Roma, Italy

Luciana Paradisi

Istituto di Patologia Generale, Corso Raffaello 30, Torino, Italy, and Istituto di Patologia Generale, Università "La Sapienza," Viale Regina Elena 324, Roma, Italy

Maurizio Parola

Istituto di Patologia Generale, Corso Raffaello 30, Torino, Italy, and Istituto di Patologia Generale, Università "La Sapienza," Viale Regina Elena 324, Roma, Italy

Luigi Frati

Istituto di Patologia Generale, Corso Raffaello 30, Torino, Italy, and Istituto di Patologia Generale, Università "La Sapienza," Viale Regina Elena 324, Roma, Italy

Mario U. Dianzani

Istituto di Patologia Generale, Corso Raffaello 30, Torino, Italy, and Istituto di Patologia Generale, Università "La Sapienza," Viale Regina Elena 324, Roma, Italy

The 4-hydroxynonenal aldehyde (HNE), a product of lipid peroxidation with high biological activity, inhibits cancerous growths in vivo and in vitro. The mechanism by which this aldehyde acts is not yet understood. The c-myc oncogene seems to be involved in the regulation of cellular multiplication and transformation. We evaluated the c-myc expression and the RNA, DNA and protein synthesis in K562 cells. These cells were incubated for 1 hour in presence of several aldehyde concentrations (range 5.10-7 to 10-4), then washed and kept for 20 hours in a growth medium until used. HNE inhibited both the nucleic acids and protein synthesis in a dose dependent manner, and c-myc expression was evaluated in the K562 cells after incubation with 10-4m or 10-6m HNE. HNE inhibited c-myc expression only at the highest dose. These preliminary results may suggest that the inhibition of c-myc expression is related to nucleic acid synthesis inhibition following HNE exposure.

  • 1. Aviv H, and Bigbee JW (1972). Purification of biologically active globin messenger RNA by chromatography on oligo-thymidylic acid cellulose column. Proc. Natl. Acad. Sci. (USA) 69: 1408–1412.[Abstract/Free Full Text]
  • 2. Blanchard JM, Bigbee JW, Bigbee JW, Bigbee JW, Bigbee JW, Bigbee JW, and Bigbee JW (1985). c-myc gene is transcribed at high rate in G0-arrested fibroblasts and is post-transcriptionally regulated in response to growth factors. Nature 317: 443–445.[CrossRef][Medline] [Order article via Infotrieve]
  • 3. Campisi J, Bigbee JW, Bigbee JW, Bigbee JW, and Bigbee JW (1984). Cell-cycle control of c-myc but not c-ras expression is lost following chemical transformation. Cell 36: 241–347.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • 4. Chirgwin JM, Bigbee JW, Bigbee JW, and Bigbee JW (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • 5. Dani C, Blanchard JM, Piechaczyk M, El Sabouty S, Bigbee JW, and Bigbee JW (1984). Extreme instability of myc mRNA in normal and transformed human cells. Proc. Natl. Acad. Sci. (USA) 81: 7046–7050.[Abstract/Free Full Text]
  • 6. Dianzani MU (1979). Biological activity of methylglyoxal and related aldehydes. In: Submolecular Biology and Cancer, Ciba Foundation Series 67. Excerpta Medica, Amsterdam, pp. 245–270.
  • 7. Dianzani MU (1982). Biological effects of aldehydes. In: Free Radicals, Lipid Peroxidation and Cancer, DCH McBrien and TF Slater (eds). Academic Press, N.Y., pp. 129–158.
  • 8. Gonda JJ, and Bigbee JW (1984). Expression of myb, myc and fos proto-oncogenes during the differentiations of a murine myeloid leukaemia. Nature 310: 249–251.[CrossRef][Medline] [Order article via Infotrieve]
  • 9. Kelly K, Bigbee JW, Bigbee JW, and Bigbee JW (1983). Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factors. Cell 35: 603–610.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • 10. Makino R, Bigbee JW, and Bigbee JW (1984). c-myc transcript is induced in rat liver at a very early stage of regeneration or by cycloheximide treatment. Nature 310: 696–697.
  • 11. Salomon DJ, Bigbee JW, Bigbee JW, and Bigbee JW (1984). Expression of cellular oncogenes in human malignancies. Science 224: 256–262.[Abstract/Free Full Text]
  • 12. White JS, and Bigbee JW (1982). Inhibitory effects of methyl glyoxal on DNA, RNA and protein synthesis in cultured guinea pig keratinocytes. Chem. Biol. Interactions 38: 339–347.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • 13. White JS, and Bigbee JW (1984). The mechanism of action of 4-hydroxynonenal in cell injury. Chem. Biol. Interactions 52: 283–241.

Toxicologic Pathology, Vol. 15, No. 2, 238-240 (1987)
DOI: 10.1177/019262338701500219
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



This Article
Right arrow Abstract Freely available
Right arrow Free Full Text (Free PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Barrera, G.
Right arrow Articles by Dianzani, M. U.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barrera, G.
Right arrow Articles by Dianzani, M. U.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?