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c-N-ras is Activated Infrequently in Canine Malignant Lymphoma

Department of Surgery, School of Veterinary Medicine, University of California, Davis. California 95616–8745

Karen A. Pazzi

Department of Surgery, School of Veterinary Medicine, University of California, Davis. California 95616–8745

Paul H. Gumerlock

Cancer and Molecular Research Laboratory, UC Davis Cancer Center, Sacramento, California 95817

Bruce R. Madewell

Department of Surgery, School of Veterinary Medicine, University of California, Davis. California 95616–8745

Activated c-N-ras alleles have been detected in human lymphoma specimens. The aim of the present study was to determine the frequency of c-N-ras mutational activation in canine malignant lymphoma. DNA was isolated from 28 canine malignant lymphoma specimens collected from 28 separate dogs and examined for c-N-ras mutations by polymerase chain reaction amplification and direct sequencing. The tumors were naturally occurring and derived from 20 dogs with known exposures to the phenoxy herbicide 2,4-dichlo-rophenoxyacetic acid (2,4-D) and from 8 dogs with no known exposure to the herbicide. An oncogenically activating mutation was found in 1 dog without known 2,4-D exposure. The mutation was a 13th codon, second position transition that would result in a glycine-to-aspartate amino acid substitution. The results of this study demonstrate that, similar to the human, c-N-ras mutations are uncommon in dogs with malignant lymphoma and that there is no association between 2,4-D exposure and activation of c-N-ras in the dog.

Key Words: ras oncogenes • 2,4-dichlorophenoxyacetic acid • polymerase chain reaction

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Toxicologic Pathology, Vol. 21, No. 3, 288-291 (1993)
DOI: 10.1177/019262339302100304


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Edwards, M. D. Articles by Madewell, B. R. Search for Related Content PubMed PubMed Citation Articles by Edwards, M. D. Articles by Madewell, B. R. Social Bookmarking                         What's this?