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Metabolic and Morphometric Changes in Small and Large Intestine in Rats Fed High-Fiber Diets
Aliza Stark
Department of Biochemistry and Human Nutrition, Faculty of Agriculture, Hebrew University, Rehovot, Israel 76100
Abraham Nyska
Koret School of Veterinary Medicine, Hebrew University of Jerusalem and Kimron Veterinary Institute, Beit Dagan, Israel
Zecharia Madar
Department of Biochemistry and Human Nutrition, Faculty of Agriculture, Hebrew University, Rehovot, Israel 76100,
Structural modifications of the intestine following fiber intake may play an important role in the physiological effects induced by dietary fiber. The aim of this study was to examine possible mechanisms for the hypocholesterolemic effects of dietary fibers by evaluating both biochemical and morphometric parameters. Thirty male Sprague-Dawley rats were divided into 3 groups and fed either a fiber-free, 15% fiber high methoxy pectin, or 15% cellulose diet for a 4-wk period. Plasma cholesterol levels, hepatic enzyme activity, and fecal bile acid excretion were determined, and changes in the intestinal structure were evaluated. Daily fecal bile acid excretion was significantly higher in rats fed the pectin diet accompanied by elevated HMG-CoA reductase activity. Pectin feeding led to significant elongation of both the small and large intestine whereas cellulose only affected colon length. Computerized image analysis of intestinal cross-sections showed enlarged muscle area in the ileum and midcolon of pectin-fed rats and greater mucosal area in the colon. Circular muscle cell hypertrophy was also observed in the colon. In contrast, the effects of cellulose feeding were limited to elongation of the colon accompanied by muscle cell hypertrophy, and little effect on ileal morphology was documented. These data indicate that the hypocholestrolemic effects of pectin may be due to increased bile acid secretion accompanied by significant morphological changes in the ileum and colon.
Key Words: Dietary fiber • pectin • cellulose • image analysis • cholesterol • tunica muscularis
- Anderson JW, Jones AE, and Riddell-Mason S. (1994). Ten different dietary fibers have significantly different effects on serum and liver lipids of cholesterol-fed rats. J. Nutr. 124: 78-83.[Abstract/Free Full Text]
- Anderson JW, Story L., Sieling B., Chen WL, Petro MS, and Story J. (1984). Hypocholesterolemic effects of oat-bran or bean intake for hypercholesterolemic men. Am. J. Clin. Nutr. 40: 1146-1155.[Abstract/Free Full Text]
- Brown MS, Goldstein JL, and Dietschy JM (1979). Active and inactive forms of 3-hydroxy-3-methylglutaryl coenzyme A reductase in the liver of the rat. J. Biol. Chem. 254: 5144-5149.[Free Full Text]
- Brown RC, Kelleher J., and Losowsky MS (1979). The effect of pectin on the structure and function of the rat small intestine. Br. J. Nutr. 42: 357-365.[CrossRef]
- Chen WL, Anderson JW, and Jenning D. (1984). Propionate may mediate the hypocholesterolemic effects of certain soluble plant fibers in cholesterol-fed rats. Proc. Soc. Exp. Biol. Med. 175: 215-218.[CrossRef][Medline]
[Order article via Infotrieve]
- Demigne C., Morand C., Moundras C., Levrat MA, Mazur A., and Remsy C. (1993). Effects of fermentable carbohydrates and SCFA on hepatic lipid metabolism. In: Cost 92-Metabolic and Physiological Aspects of Dietary Fibre in Food-Mechanisms of Action of Dietary Fibre on Lipid and Cholesterol Metabolism, D. Lairon (ed). Commission of the European Communities, Luxembourg, pp. 107-110.
- Eastwood MA (1992). The physiological effect of dietary fiber: An update. Annu. Rev. Nutr. 12: 19-35.[CrossRef][Web of Science][Medline]
[Order article via Infotrieve]
- Ebihara K., Miyada T., and Nakajima A. (1993). Hypocholesterolemic effect of cecally infused propionic acid in rats fed a cholesterol-free, casein diet. Nutr. Res. 13: 209-217.[CrossRef][Web of Science]
- Folch J., Lees M., and Sloan-Stanley GH (1957). A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497-509.[Free Full Text]
- Gallaher DD, Hassel CA, Lee K., and Gallaher CM (1993). Viscosity and fermentability as attributes of dietary fiber responsible for the hypocholesterolemic effect in hamsters. J. Nutr. 123: 244-252.[Abstract/Free Full Text]
- Jacobs LR (1985). Differential effects of dietary fibers on rat intestinal circular muscle cell size. Dig. Dis. Sci. 30: 247-252.[CrossRef][Web of Science][Medline]
[Order article via Infotrieve]
- Jenkins DJA, Wolever TMS, Leeds AR, Gassull MA, Haisman D., Dilawari J., Goff D., Metz GL, and Alberti Kgmm (1978). Dietary fibres, fibre analogues and glucose tolerance: Importance of viscosity. Br. Med. J. 1: 1392-1394.[Abstract/Free Full Text]
- Judd PA and Truswell AS (1985). The hypocholesterolemic effects of pectins in rats. Br. J. Nutr. 53: 409-425.[CrossRef]
- Kripke SA, Fox AD, Berman JM, Settle RG, and Rombeau JL (1989). Stimulation of intestinal mucosal growth with intracolonic infusion of short-chain fatty acids. J. Parenteral Enteral Nutr. 13: 109-116.[Abstract/Free Full Text]
- Kritchevsky D. (1988). Dietary fiber. Annu. Rev. Nutr. 8: 301-328.[CrossRef]
- Nagengast FM (1992). Dietary fibre and bile acid metabolism. In: Dietary Fibre-A Component of Food, Nutritional Function in Health and Disease, TF Schweizer and CA Edwards (eds). Springer-Verlag, London, pp. 217-231.
- Nishina PM and Freedland RA (1990). Effects of propionate on lipid biosynthesis in isolated rat hepatocytes. J. Nutr. 120: 668-673.[Abstract/Free Full Text]
- Nishina PM and Freedland RA (1990). The effects of dietary fiber feeding on cholesterol metabolism in rats. J. Nutr. 120: 800-805.[Abstract/Free Full Text]
- Read NW and Eastwood MA (1992). Gastro-intestinal physiology and function. In: Dietary Fibre-A Component of Food, Nutritional Function in Health and Disease, TF Schweizer and CA Edwards (eds). Springer-Verlag, London, pp. 103-117.
- Ross JK and Leklem JE (1981). The effect of dietary citrus pectin on the excretion of human fecal neutral and acid steroids and the activity of 7
 -dehydroxylase and β-glucuronidase. Am. J. Clin. Nutr. 34: 2068-2077.[Abstract/Free Full Text] - Sandberg A., Ahderinne R., Andersson H., Hallgren B., and Hulten L. (1983). The effect of citrus pectin on the absorption of nutrients in the small intestine. Hum. Nutr.: Clin. Nutr. 37C: 171-183.[Web of Science][Medline]
[Order article via Infotrieve]
- Schneeman BO and Richter D. (1993). Changes in plasma and hepatic lipids, small intestinal histology and pancreatic enzyme activity due to aging and dietary fiber in rats. J. Nutr. 123: 1328-1337.[Abstract/Free Full Text]
- Slavin G., Sowter C., Robetson K., Mcdermott S., and Paton K. (1980). Measurement in jejunal biopsies by computer-aided microscopy. J. Clin. Pathol. 33: 254-261.[Abstract/Free Full Text]
- Stark A. and Madar Z. (1993). In vitro production of short-chain fatty acids by bacterial fermentation of dietary fiber compared with effects of those fibers on hepatic sterol synthesis in rats. J. Nutr. 123: 2166-2173.[Abstract/Free Full Text]
- Stark A., Nyska A., Zuckerman A., and Madar Z. (1995). Changes in intestinal tunica muscularis following dietary fiber feeding in rats- A morphometric study using image analysis. Digestive Dis. Sci. 40: 960-966.[CrossRef]
- Superko HR, Haskell WL, Sawrey-Kubicek L., and Farquhar JW (1988). Effects of solid and liquid guar gum on plasma cholesterol and triglyceride concentrations in moderate hypercholesterolemia. Am. J. Cardiol. 62: 51-55.[CrossRef][Web of Science][Medline]
[Order article via Infotrieve]
- Thomas JN, Kelley MJ, and Story JA (1984). Alteration of regression of cholesterol accumulation in rats by dietary pectin. Br. J. Nutr. 51: 339-345.[CrossRef]
- Topping DL, Ilman RJ, Dowling K., and Trimble RP (1990). Mechanisms whereby fibre could lower plasma cholesterol. In: Dietary Fibre: Chemical and Biological Aspects, DAT Southgate, K Waldron, IT Johnson, and GR Fenwick (eds). The Royal Society of Chemistry, Cambridge, pp. 300-304.
- Truswell AS and Beynen AC (1992). Dietary fibre and plasma lipids: Potential for prevention and treatment of hyperlipidaemias. In: Dietary Fibre-A Component of Food, Nutritional Function in Health and Disease, TF Schweizer and CA Edwards (eds). Springer-Verlag, London, pp. 295-332.
- Vahouny GV, Roy T., Gallo LL, Story JA, Kritchevsky D., and Cassidy M. (1980). Dietary fibers III. Effects of chronic intake on cholesterol absorption and metabolism in the rat. Am. J. Clin. Nutr. 33: 2182-2191.[Free Full Text]
- Wrick KL, Robertson JB, Van Soest PJ, Lewis BA, Rivers JM, Roe DA, and Hackler LR (1983). The influence of dietary fiber source on human intestinal transit and stool output. J. Nutr. 113: 1464- 1479.[Abstract/Free Full Text]
- Wright RS, Anderson JW, and Bridges SR (1990). Propionate inhibits hepatocyte lipid synthesis. Proc. Soc. Exp. Biol. Med. 195: 26-29.[CrossRef][Medline]
[Order article via Infotrieve]
Toxicologic Pathology, Vol. 24, No. 2,
166-171 (1996)
DOI: 10.1177/019262339602400204
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