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Alterations in Glomerular Anionic Sites in the Autologous Phase of Canine Anti-Glomerular Basement Membrane Nephritis

Mitsubishi Chemical Corporation, Yokohama Research Center, Yokohama 227, Japan

Masahiko Wasaki

Mitsubishi Chemical Corporation, Yokohama Research Center, Yokohama 227, Japan

Yoshimi Inoue

Mitsubishi Chemical Corporation, Yokohama Research Center, Yokohama 227, Japan

Kinji Shirota

Azabu Univercity, Kanagawa 229, Japan

Yasuo Nomura

Azabu Univercity, Kanagawa 229, Japan

There have been a few studies on canine nephrotoxic glomerulonephritis produced by anti-glomerular basement membrane serum (AGBM), but these reports have not focused on an alteration in the charge properties of glomerular basement membrane (GBM). In this study, rabbit AGBM or normal rabbit serum (NRS) was given intravenously (2 ml/kg body weight) to 16 male beagle dogs. An alteration of anionic sites (ASs) of GBM was studied quantitatively using polyethyleneimine as a cationic probe by electron microscopy at weeks 1, 2, 4, and 8 postinjection. In AGBM-treated dogs, severe or mild proteinuria continued until week 2. At weeks 4 and 8, there was no significant difference in the intensity of proteinuria between AGBM- and NRS-treated groups. Until week 2 postinjection, there were significantly fewer ASs of GBM in AGBM-treated dogs than in NRS-treated dogs. At week 8, however, there was no difference in ASs of GBM between AGBM- and NRS-treated dogs. The fact that a reduction of glomerular AS occurred in AGBM-treated dogs with severe or mild proteinuria and the recovery of AS in the GBM coincided with an improvement of proteinuria suggested that alteration of the glomerular ASs might play an important role in the pathogenesis of proteinuria in canine anti-GBM nephritis.

Key Words: Anionic sites • anti-glomerular basement membrane serum • glomerulonephritis • polyethyleneimine

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Toxicologic Pathology, Vol. 24, No. 5, 627-635 (1996)
DOI: 10.1177/019262339602400514


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Sugimoto, J. Articles by Nomura, Y. Search for Related Content PubMed PubMed Citation Articles by Sugimoto, J. Articles by Nomura, Y. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Social Bookmarking                         What's this?