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Revolution through Genomics in Investigative and Discovery Toxicology
Charles P. Rodi
Genomic Sequencing Center, Monsanto Life Sciences Company, St. Louis, Missouri 63198, charles.p.rodi @ monsanto.com.
Roderick T. Bunch
Monsanto Safety Evaluation, G.D. Searle/Monsanto, Skokie, Illinois 60077
Sandra W. Curtiss
Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167
Larry D. Kier
Nutrition Sector, Monsanto Life Sciences Company, St. Louis, Missouri 63167
Marc A. Cabonce
Nutrition Sector, Monsanto Life Sciences Company, St. Louis, Missouri 63167
Julio C. Davila
Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167
Michael D. Mitchell
Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167
Carl L. Alden
Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167
Dale L. Morris
Monsanto Safety Evaluation, G.D. Searle/Monsanto, St. Louis, Missouri 63167
The remarkable technologic and methodologic advances spurred on by the Human Genome Project are being applied throughout the life sciences. In the field of toxicology, high-resolution assays now make it possible to discover virtually all the differences in gene expression brought on by exposure to a particular xenobiotic. There are 2 principal approaches used to build a catalog of changes in gene expression: hybridization microarrays and gel-based methods, such as differential display and AFLPTM-based mRNA fingerprinting. The power of such approaches is exemplified by the identification of more than 300 genes that differ in expression level by at least 2-fold in response to the nongenotoxic rodent liver carcinogen phenobarbital.
Key Words: Genomics • toxicology • gene expression • phenobarbital • liver carcinogen
References
- Choi SY and Park OJ (1996). Differential display analysis of gene expression induced under DCA treatment in rat liver. J. Biochem. Mol. Biol. 29: 272-275.[Web of Science]
- Liang P. and Pardee AB (1992). Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257: 967-971.[Abstract/Free Full Text]
- Lipshutz RJ, Morris D., Chee M., Hubbell E., Kozal MJ, Shah N., Shen N., Yang R., and Fodor Spa (1995). Using oligonucleotide probe arrays to access genetic diversity. BioTechniques 19: 442-447.[Web of Science][Medline]
[Order article via Infotrieve]
- Marshall A. and Hodgson J. (1998). DNA chips: An array of possibilities. Nat. Biotechnol. 16: 27-32.[CrossRef][Web of Science][Medline]
[Order article via Infotrieve]
- Money T., Reader S., Qug LJ, Dunford RP, and Moore G. (1996). AFLP-based mRNA fingerprinting. Nucleic Acids Res. 24: 2616-2617.[Free Full Text]
- Russell ME, Utans U., Wallace AF, Liang P., Arceci RJ, Karnovsky MJ, Wyner LR, Yamashita Y., and Tarn C. (1994). Identification and upregulation of galactose/N-acetylgalactosamine macrophage lectin in rat cardiac allografts with arteriosclerosis. J. Clin. Invest. 94: 722-730.[Web of Science][Medline]
[Order article via Infotrieve]
- Schena M., Shalon D., Davis RW, and Brown PO (1995). Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 270: 467-470.[Abstract/Free Full Text]
- Schena M., Shalon D., Heller R., Chai A., Brown PO, and Davis RW (1996). Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes. Proc. Natl. Acad. Sci. USA 93: 10614-10619.[Abstract/Free Full Text]
- Shalon D., Smith SJ, and Brown PO (1996). A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization. Genome Res. 6: 639-645.[Abstract/Free Full Text]
- Southern EM, Case-Green SC, Elder JK, Johnson M., Mir KU, Wang L., and Williams JC (1994). Arrays of complementary oligonucleotides for analysing the hybridisation behaviour of nucleic acids. Nucleic Acids Res. 22: 1368-1373.[Abstract/Free Full Text]
- Southern EM, Maskos U., and Elder JK (1992). Analyzing and comparing nucleic acid sequences by hybridization to arrays of oligonucleotides: Evaluation using experimental models. Genomics 13: 1008-1017.[CrossRef][Web of Science][Medline]
[Order article via Infotrieve]
- Watson MA and Fleming TP (1994). Isolation of differentially expressed sequence tags from human breast cancer. Cancer Res. 54: 4598-4602.[Abstract/Free Full Text]
Toxicologic Pathology, Vol. 27, No. 1,
107-110 (1999)
DOI: 10.1177/019262339902700120
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