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Toxicologic Pathology
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Eosinophilic Gastroenterocolitis in Iron Lactate-Overloaded Rats

Isao Narama

Research Institute of Drug Safety, Setsunan University, Hirakata, Osaka 573-01, Japan

Kiyokazu Ozaki

Research Institute of Drug Safety, Setsunan University, Hirakata, Osaka 573-01, Japan

Syuuichi Matsushima

Research Institute of Drug Safety, Setsunan University, Hirakata, Osaka 573-01, Japan, Developmental Research Setsunan University, Co., Toyonaka, 573-01, Japan

Tetsuro Matsuura

Research Institute of Drug Safety, Setsunan University, Hirakata, Osaka 573-01, Japan

Eosinophilic gastroenterocolitis with peripheral eosinophilia was induced in rats fed a diet containing 2.5% or 5.0% iron lactate for 3 mo. Additional findings consistent with iron overload were also observed. Microscopically, the lesions consisted of eosinophilic infiltrations in the mucosa and submucosa along the whole length of the gastrointestinal tracts, increased surface area of the gastric mucosal propria covered with mucous cells, and increased apoptotic bodies in the gastric glandular neck of rats in the 2.5% and 5.0% groups. An increased number of intraepithelial globule leukocytes in the gastric and intestinal lamina propria was also observed in the 5.0% group. Globule leukocytes in the gastric mucosa contained obviously enlarged granules in their cytoplasm in these rats. The granules of the globule leukocytes were positive for rat mast cell protease II, suggesting the mastocyte origin of these cells. Although severe infiltration of eosinophils and globule leukocytes suggested a type-1 hypersensitivity reaction, other features such as an increasing vascular permeability were not detected. Serum IgE levels in the 5.0% and control groups were <3 ng/ml. Final body weights of male and female rats of the 5.0% group were suppressed to 70% and 90%, respectively, of those of the control rats, whereas food consumption was comparable to that of the control group. The morphologic characteristics of the gastrointestinal lesions and peripheral eosinophilia induced in rats fed iron lactate were very similar to those in some cases of eosinophilic gastroenterocolitis in humans and other animals.

Key Words: Eosinophilia • eosinophilic infiltration • gastritis • enteritis • colitis • globule leukocyte

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Toxicologic Pathology, Vol. 27, No. 3, 318-324 (1999)
DOI: 10.1177/019262339902700307
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