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Ito Cell Tumor: Immunohistochemical Investigations of a Rare Lesion in the Liver of Mice
Thomas Tillmann
Institut für Experimentelle Pathologie, Medizinische Hochschule Hannover, D-30625 Hannover, Germany
Kenji Kamino
Institut für Experimentelle Pathologie, Medizinische Hochschule Hannover, D-30625 Hannover, Germany
Clemens Dasenbrock
Fraunhofer Institut fiir Toxikologie und Aerosolforschung, D-30625 Hannover, Germany
Paul G. Germann
Byk Gulden, Institut für Pathologie und Toxikologie, D-22047 Hamburg, Germany
Manfred Kohler
Fraunhofer Institut fiir Toxikologie und Aerosolforschung, D-30625 Hannover, Germany
Gerd Morawietz
Fraunhofer Institut fiir Toxikologie und Aerosolforschung, D-30625 Hannover, Germany
Elias Campo
Departamento de Anatomia Patologica, Hospital Clinic, E-8036 Barcelona, Spain
Antonio Cardesa
Departamento de Anatomia Patologica, Hospital Clinic, E-8036 Barcelona, Spain
Lorenzo Tomatis
Istituto per L'Infanzia, I-34137 Trieste, Italy
Ulrich Mohr
Institut für Experimentelle Pathologie, Medizinische Hochschule Hannover, D-30625 Hannover, Germany
In 2 lifespan transgeneration experiments using a total of 4,682 CBA/J mice, we observed uncommon lipomatous lesions in the livers of 8 mice independent of the treatment. Macroscopically, the lesions were described as pale white areas (2) or nodules (6) during necropsy. The lesions ranged from 1 to 15 mm in diameter. Microscopically, the lesions consisted of nodular aggregations of round to spindle-shaped cells that partly caused distinct compression of the adjacent hepatic parenchyma. The tumor cells were smaller than hepatocytes and had dark oval nuclei. Many of the more spherical cells contained clear vacuoles of various sizes, which were shown to be lipid droplets by oil red O staining. In addition to Gomori's silver and Masson's trichrome staining, several immunohistochemical stains were used to characterize the origin of the proliferating cells. Tumor cells were labeled by vimentin, actin, desmin, and proliferating cell nuclear antigen. The 2 cell phenotypes showed similar staining characteristics. Increased amounts of laminin and tenascin, 2 extracellular matrix proteins of the liver, were detected within these neoplasms. Summarizing, we suggest that these tumors are of Ito cell origin.
Key Words: Liver neoplasms • perisinusoidal cells • Ito cells • fat-storing cells • stellate cells • histomorphology • mice • inbred CBA
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Toxicologic Pathology, Vol. 27, No. 3,
364-369 (1999)
DOI: 10.1177/019262339902700314
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