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Quantitative Analysis of the Lobular Distribution of S-Phase in Rat Liver Following Dietary Administration of Di(2-ethylhexyl)phthalate

Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, United Kingdom

Suzanne Cliffe

Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, United Kingdom

Ian Pate

Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, United Kingdom

John R. Foster

Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, United Kingdom

A simple image-analysis method is described, whereby the distribution of hepatocytes that have entered S-phase, as distinguished by the incorporation of bromodeoxyuridine, can be related to the position of the central and portal veins of the hepatic lobule. Hepatocyte S-phase was induced in the livers of male and female F344 rats by administration of di(2-ethylhexyl)phthalate (DEHP) in the diet for 7 days at 2 dose levels, and these livers were used to develop the procedure. The distributions of the S-phase between control and DEHP-treated livers were compared using statistical techniques. The results showed notable differences in the distribution of S-phase between male and female rats as well as limited dose-related effects.

Key Words: Carcinogenic prediction • di(2-ethylhexyl)phthalate • hepatocyte S-phase • quantitative lobular distribution • rat

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Toxicologic Pathology, Vol. 27, No. 4, 436-440 (1999)
DOI: 10.1177/019262339902700407


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Soames, A. R. Articles by Foster, J. R. Search for Related Content PubMed PubMed Citation Articles by Soames, A. R. Articles by Foster, J. R. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB BIS(2-ETHYLHEXYL)PHTHALATE Medline Plus Health Information Diets Social Bookmarking                         What's this?