This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ogawa, K. Articles by Cohen, S. M. Search for Related Content PubMed PubMed Citation Articles by Ogawa, K. Articles by Cohen, S. M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information Bladder Cancer Social Bookmarking                         What's this?

Comparison of Uroplakin Expression During Urothelial Carcinogenesis Induced by N-Butyl-N-(4-Hydroxybutyl)Nitrosamine in Rats and Mice

Department of Pathology and Microbiology and the Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, Nebraska 68198-3135, USA, First Department of Pathology, Nagoya City University Medical School, Nagoya 454, Japan

Margaret St. John

Department of Pathology and Microbiology and the Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, Nebraska 68198-3135, USA

Maria Luiza De Oliveira

Department of Pathology and Microbiology and the Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, Nebraska 68198-3135, USA

Lora Arnold

Department of Pathology and Microbiology and the Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, Nebraska 68198-3135, USA

Tomoyuki Shirai

First Department of Pathology, Nagoya City University Medical School, Nagoya 454, Japan

Tung-Tien Sun

Epithelial Biology Unit, The Ronald O. Perelman Department of Dermatology, New York University School of Medicine, New York, New York, 10016, USA, Department of Pharmacology and Kaplan Comprehensive Cancer Center, New York University School of Medicine, New York, New York 10016, USA

Samuel M. Cohen

Department of Pathology and Microbiology and the Eppley Institute for Research in Cancer, University of Nebraska Medical Center, Omaha, Nebraska 68198-3135, USA, scohen{at}unmc.edu.

The expression of uroplakins, the tissue-specific and differentiation-dependent membrane proteins of the urothelium, was analyzed immunohistochemically in N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN)-treated rats and mice during bladder carcinogenesis. Male Fischer 344 rats were treated with 0.05% BBN in the drinking water for 10 wk and were euthanatized at week 20 of the experiment. BBN was administered to male B6D2F₁ mice; it was either provided at a rate of 0.05% in the drinking water (for 26 wk) or 5 mg BBN was administered by intragastric gavage twice weekly for 10 wk, followed by 20 wk without treatment. In rats, BBN-induced, noninvasive, low-grade, papillary, transitional cell carcinoma (TCC) showed decreased uroplakin-staining of cells lining the lumen but showed increased expression in some nonluminal cells. In mice, nonpapillary, high-grade dysplasia, carcinoma in situ, and invasive carcinoma were induced. There was a marked decrease in the number of uroplakin-positive cells lining the lumen and in nonluminal cells. This occurred in normal-appearing urothelium in BBN-treated mice and in dysplasic urothelium, in carcinoma in situ, and in invasive TCC. The percentage of uroplakin-positive nonluminal cells was higher in control mice than in rats, but it was lower in the mouse than in the rat after BBN treatment. Uroplakin expression was disorderly and focal in BBN-treated urothelium in both species. These results indicate that BBN treatment changed the expression of uroplakins during bladder carcinogenesis, with differences in rats and mice being related to degree of tumor differentiation.

Key Words: Uroplakins • differentiation • bladder cancer • cell membranes

References

• Arai M., Kani T., Sugihara S., Matsumura K., Miyata Y., Shinohara Y., and Ito N. (1974). Scanning and transmission electron microscopy of changes in the urinary bladder in rats treated with N-butyl-N-(4-hydroxybutyl)nitrosamine. Gann 65: 529-540.[Web of Science][Medline] [Order article via Infotrieve]
• Becci PJ, Thompson HJ, Strum JM, Brown CC, Sporn MB, and Moon RC (1981). N-butyl-N-(4-hydroxybutyl)nitrosamine-induced urinary bladder cancer in C57BL/6 X DBA/2F1 mice as a useful model for study of chemoprevention of cancer with retinoids. Cancer Res. 41: 927-932.[Abstract/Free Full Text]
• Cohen SM (1983). Pathology of experimental bladder cancer in rodents. In: The Pathology of Bladder Cancer, Vol. 2, GT Bryan and SM Cohen (eds), CRC Press, Boca Raton, Florida, pp. 1-40.
• Cohen SM, Ellwein LB, Okamura T., Masui T., Johansson SL, Smith RA, Wehner JM, Khachab M., Chappel CI, Schoenig GP, Emerson JL, and Garland EM (1991). Comparative bladder tumor promoting activity of sodium saccharin, sodium ascorbate, related acids, and calcium salts in rats. Cancer Res. 51: 1766-1777.[Abstract/Free Full Text]
• Ertürk E., Cohen SM, and Bryan GT (1970). Urinary bladder carcinogenicity of N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide in female Swiss mice. Cancer Res. 30: 1309-1311.[Abstract/Free Full Text]
• Ertürk E., Price JM, Morris Je, Cohen S, Leith Rs, Von Esch Am, and Crovetti AJ (1967). The production of carcinoma of the urinary bladder in rats by feeding N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide. Cancer Res. 27: 1998-2002.[Abstract/Free Full Text]
• Hsu SM, Raine L., and Fanger H. (1981). Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29: 577-580.[Abstract]
• Ito N., Hiasa Y., Tamai A., Okajima E., and Kitamura H. (1969). Histogenesis of urinary bladder tumors induced by N-butyl-N-(4-hydroxybutyl)nitrosamine in rats. Gann 60: 401-410.[Web of Science][Medline] [Order article via Infotrieve]
• Jacobs JB, Arai M., Cohen SM, and Friedell GH (1976). Early lesions in experimental bladder cancer: Scanning electron microscopy of cell surface markers. Cancer Res. 36: 2512-2517.[Abstract/Free Full Text]
• Lin J-H., Wu X-R., Kreibich G., and Sun T-T. (1994). Precursor sequence, processing, and urothelium-specific expression of a major 15-kDa protein subunit of asymmetric unit membrane. J. Biol. Chem. 269: 1775-1784.[Abstract/Free Full Text]
• Lin J-H., Zhao H., and Sun T-T. (1995). A tissue-specific promoter that can drive a foreign gene to express in the suprabasal urothelial cells of transgenic mice. Proc. Natl. Acad. Sci. USA 92: 679-683.[Abstract/Free Full Text]
• Martin RA, Daly AM, Difonzo CJ, and de la Iglesia FA (1984). Randomization of animals by computer program for toxicity studies. J. Am. Coll. Toxicol. 3: 1-11.[CrossRef]
• Ogawa K., Sun T-T., and Cohen SM (1996). Analysis of differentiation associated proteins in rat bladder carcinogenesis. Carcinogenesis 17: 961-965.[Abstract/Free Full Text]
• SAS Institute, Inc. (1989 ). SAS/STAT User's Guide, version 6, Vol. 2, 4th ed. SAS Institute, Inc., Cary, North Carolina.
• Walz T., Häner M., Wu X-R. Henn C., Engel A., Sun T-T., and Aebi U. (1995). Towards the molecular architecture of the asymmetric unit membrane of the mammalian urinary bladder epithelium: A closed "twisted ribbon" structure. J. Mol. Biol. 248: 887-900.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Wu X-R., Lin J-H., Walz T., Häner M., Yu J., Aebi U., and Sun T-T. (1994). Mammalian uroplakins; A group of highly conserved urothelial differentiation-related membrane proteins. J. Biol. Chem. 269: 13716-13724.[Abstract/Free Full Text]
• Wu X-R., Manabe M., Yu J., and Sun T-T. (1990). Large scale purification and immunolocalization of bovine uroplakins I, II and III. J. Biol. Chem. 265: 19170-19179.[Abstract/Free Full Text]
• Wu X-R. and Sun T-T. (1993). Molecular cloning of a 47 kDa tissue-specific and differentiation-dependent urothelial cell surface glycoprotein. J. Cell Sci. 106: 31-43.[Abstract]
• Yu J., Lin J-H., Wu X-R., and Sun T-T. (1994). Uroplakins Ia and Ib, two major differentiation products of bladder epithelium, belong to a family of four transmembrane domain (4TM) proteins. J. Cell Biol. 125: 171-182.[Abstract/Free Full Text]
• Yu J., Manabe M., Wu X-R., Xu C., Surya B., and Sun T-T. (1990). Uroplakin I: A 27-kDa protein associated with the asymmetric unit membrane of mammalian urothelium. J. Cell Biol. 111: 1207-1216.[Abstract/Free Full Text]

Toxicologic Pathology, Vol. 27, No. 6, 645-651 (1999)
DOI: 10.1177/019262339902700606


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. A. Ramos-Vara, M. A. Miller, M. Boucher, A. Roudabush, and G. C. Johnson Immunohistochemical Detection of Uroplakin III, Cytokeratin 7, and Cytokeratin 20 in Canine Urothelial Tumors Vet. Pathol., January 1, 2003; 40(1): 55 - 62. [Abstract] [Full Text] [PDF]

				V. Ambrosio, G. Borzacchiello, F. Bruno, P. Galati, and F. Roperto Uroplakin Expression in the Urothelial Tumors of Cows Vet. Pathol., November 1, 2001; 38(6): 657 - 660. [Abstract] [Full Text] [PDF]

This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ogawa, K. Articles by Cohen, S. M. Search for Related Content PubMed PubMed Citation Articles by Ogawa, K. Articles by Cohen, S. M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information Bladder Cancer Social Bookmarking                         What's this?