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Nedocromil Sodium Inhibits Canine Adenovirus Bronchiolitis in Beagle Puppies
Kurt A. Anderson
Department of Pediatrics, Steele Memorial Children's Research Center, Department of Pediatrics, Respiratory Sciences Center, and Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona 85724
Richard J. Lemen
Department of Pediatrics, Steele Memorial Children's Research Center, Department of Pediatrics, Respiratory Sciences Center, and Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona 85724, rlemen{at}umcaz.edu
Natalie S. Weger
Department of Pediatrics, Steele Memorial Children's Research Center, Department of Pediatrics, Respiratory Sciences Center, and Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona 85724
Huizhong Chen
Department of Pediatrics, Steele Memorial Children's Research Center, Department of Pediatrics, Respiratory Sciences Center, and Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona 85724
Mark C. Bowers
Department of Pediatrics, Steele Memorial Children's Research Center, Department of Pediatrics, Respiratory Sciences Center, and Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona 85724
Mark L. Witten
Department of Pediatrics, Steele Memorial Children's Research Center, Department of Pediatrics, Respiratory Sciences Center, and Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona 85724
R. Clark Lantz
Department of Pediatrics, Steele Memorial Children's Research Center, Department of Pediatrics, Respiratory Sciences Center, and Department of Cell Biology and Anatomy, University of Arizona, Tucson, Arizona 85724
David E. Bice
The Lovelace Respiration Institute, Albuquerque, New Mexico 87111
Bruce A. Muggenburg
The Lovelace Respiration Institute, Albuquerque, New Mexico 87111
Nedocromil sodium is a nonsteroidal anti-inflammatory drug used to control asthmatic attacks. Our hypothesis is that nedocromil sodium inhibits virus-induced airway inflammation, a common trigger of asthma. We nebulized nedocromil sodium into beagle dogs (n = 10, mean ± SEM ages: 149 ± 13 days) before and after inoculation with canine adenovirus type 2 (CAV2). Control dogs (n = 10) received saline aerosols and were either infected with CAV2 (Sa1/CAV2, n = 7, mean ± SEM ages: 140 ± 11 days) or were not infected (Sal/Sal, n = 3, ages: 143 ± 0 days). All dogs were anesthetized with choralose (80 mg/kg IV), intubated, and mechanically ventilated. Pulmonary function tests and bronchoalveolar lavage (BAL) were performed using standard techniques. Pulmonary function tests revealed no significant change between the nedocromil sodium and non-nedocromil-treated groups. The percentage of infected bronchioles was quantitated as the number of inflamed airways of 40 bronchioles examined times 100 for each dog. Nedocromil-treated dogs had significantly (p < 0.05) less mucosal inflammation (mean ± SEM, 39% ± 5%), epithelial denudation (36% ± 5%), and BAL neutrophilia (11 ± 3) than did Sal/CAV2 dogs (51% ± 6%, 57% ± 4%, and 33% ± 8%, respectively). We concluded that pretreatment with nedocromil sodium aerosols attenuated CAV2-induced airway inflammation in these beagle puppies.
Key Words: Nedocromil sodium • bronchiolitis • viral infections • inflammation • pulmonary function
References
- Becker S., Quay J., Soukup J. (1991). Cytokine (tumor necrosis factor, IL-6, and IL-8) production by respiratory syncytial virus-infected human alveolar macrophages. J Immunol 147: 4307-4312.[Abstract]
- Borson DB, Brokaw JJ, Sekizawa K., McDonald DM, Nadel JA (1989). Neutral endopeptidase and neurogenic inflammation in rats with respiratory infections. J Appl Physiol 66: 2653-2658.[Abstract/Free Full Text]
- Busse WW, Randlev B., Arbabian M., Ruoho A. (1989). Effect of nedocromil sodium on the β-adrenergic response of human polymorphonuclear leococytes. Drugs 37(suppl 1): 26-31.[Web of Science][Medline]
[Order article via Infotrieve]
- Chitano P., Finotto S., Basco V., Mapp C., Fabbri LM (1987). Nedocromil sodium inhibits bronchoalveolar lavage neutrophilia induced by ozone in dogs. In: The Lung and the Environment, Stam J, Siebelink J, Vanderschweren R, Wagenaar J (eds). CIP Gegerens Konilijke Bibliotheck, The Hague, pp 325-331.
- Cunningham JC, Morgan WJ, Lemen RJ, Witten ML, Magarelli JL, Quan SF (1987). Passive exhalation technique correlates with esophageal balloon measurements of respiratory mechanics in beagle puppies. Am Rev Respir Dis 136: 722-726.[Web of Science][Medline]
[Order article via Infotrieve]
- Dambro NN, Grad R., Witten ML, Quan SF, Sobonya RE, Ray GC, Devine L., Lemen RJ (1992). Bronchoalveolar lavage fluid cytology reflects airway inflammation in beagle puppies with acute bronchiolitis. Pediatr Pulmonol 12: 213-220.[CrossRef][Web of Science][Medline]
[Order article via Infotrieve]
- Faden H., Hong JJ, Ogra PL ( 1984). Interaction of polymorphonuclear leukocytes and viruses in humans: Adherence of polymorphonuclear leukocytes to respiratory syncytial virus-infected cells. J Virol 52: 16-23.[Abstract/Free Full Text]
- Grad R., Sobonya RE, Witten ML, Quan SF, Ray CG, Devine LC, Lentz LA, Lemen RJ (1990). Localization of inflammation and virions in canine adenovirus type 2 bronchiolitis. Am Rev Respir Dis 142: 691-699.[Web of Science][Medline]
[Order article via Infotrieve]
- Grad R., Witten ML, Quan SF, McKelvie DH, Lemen RJ (1988). Intravenous Chloralose is a safe anesthetic for longitudinal use in beagle puppies. Lab Anim Sci 38: 422-425.[Web of Science][Medline]
[Order article via Infotrieve]
- Hers Jfph (1966). Disturbances of the ciliated epithelium due to influenza virus. Am Rev Respir Dis 93: 162-171.[Web of Science][Medline]
[Order article via Infotrieve]
- Jacoby BD, Tamaolki J., Borson DS, Nadel JA (1988). Influenza infection increases airway smooth muscle responsiveness to substance P in ferrets by decreasing enkephalinase. J Appl Physiol 64: 2653-2658.[Abstract/Free Full Text]
- Konig P., Eigen H., Ellis MH, Ellis E., Blake K., Geller D., Shapiro G., Welch M., Scott C. (1995). The effect of nedocromil sodium on childhood asthma during the viral season. Am J Respir Crit Care Med 152: 1879-1886.[Abstract]
- Lantz RC, Witten ML, Lemen RJ (1997). Pulmonary macrophage and respiratory virus interactions. In: Lung Macrophage and Dendritic Cells in Health and Disease, Lipscomb MF, Russel SW (eds.). Marcel Dekker, New York, pp 551-570. Lung Biology in Health and Disease, Vol 20, Lenfant C (exec. ed.).
- Lemen RJ (1990). Clues to the mechanisms of virus-induced asthma from animal models. Semin Respir Med 11: 321-329.
- Lemen RJ, Quan SF, Witten ML, Sobonya RE, Ray CG, Grad R. ( 1990). Canine parainfluenza type 2 bronchiolitis increases histamine responsiveness in beagle puppies. Am Rev Respir Dis 141: 199-207.[Web of Science][Medline]
[Order article via Infotrieve]
- Leung Kbp, Flint KC, Brostoff J., Hudspith BN, Johnson NM, Pearce FL (1989). A comparison of nedocromil sodium and sodium cromoglycate on human lung mast cells obtained by bronchoalveolar lavage and by dispersion of lung fragments. Eur J Respir Dis 69(suppl 147): 223-226.
- Moqbel R., Cromwell O., Walsh GM, Wardlaw AJ, Kurlak L., Kay AB ( 1988). Effects of nedocromil sodium (Tilade®) on the activation of human eosinophils and neutrophils and the release of histamine from mast cells. Allergy 43: 268-276.[Web of Science][Medline]
[Order article via Infotrieve]
- Muggenburg BA, Hoover MD, Gruffith BP, Haley PJ, Snipes MB,
- Wolff RK, Yeh HC, Burckart GJ, Mauderly JL (1990). Administration of cyclosporin by inhalation: A feasibility study in beagle dogs. J Aersol Med 3: 1-15.
- Prince GA, Porter DD (1996). Treatment of parainfluenza virus type 3 bronchiolitis and pnuemonia in a cotton rat model using antibody and glucocortcoid. J Infect Dis 173: 598-608.[Web of Science][Medline]
[Order article via Infotrieve]
- Quan SF, Lemen RJ, Witten ML, Sherrill DL, Grad R., Sobonya RE, Ray CG ( 1990). Lung mechanics and reactivity with age and after viral bronchiolitis in beagle puppies. J Appl Physiol 69: 2034-2042.[Abstract/Free Full Text]
- Quan SF, Witten ML, Dambro N., Lemen RJ (1991). Canine parainfluenza type 2 and Bordetella bronchiseptica infection produces increased bronchoalveolar lavage thromboxane concentrations in beagle puppies. Prostaglandins Leukotrienes Essent Fatty Acids 44: 171-175.[CrossRef][Web of Science][Medline]
[Order article via Infotrieve]
- Quan SF, Witten ML, Grad R., Sobonya RE, Ray CG, Dambro NN, Lemen RJ (1990). Acute canine adenovirus 2 infection increases histamine airway reactivity in beagle puppies. Am Rev Respir Dis 141: 414-420.[Web of Science][Medline]
[Order article via Infotrieve]
- Smith JJ, Lemen RJ, Taussig LM (1987). Mechanisms of viral induced lower airway obstruction. Pediatr Infect Dis J 6: 837-842. 24. Vanhotte PM (1988). Epithelium-derived relaxing factors and bronchiole reactivity. Am Rev Respir Dis 138: S24-S30.[Web of Science][Medline]
[Order article via Infotrieve]
- Witten M., Bowers MC, Hall JN, Quan SF, Chen H., Lemen RJ (1992). A rapid analytical method for measuring drug distribution in aerosols. J Nucl Med Technol 20: 155-158.
Toxicologic Pathology, Vol. 28, No. 2,
317-325 (2000)
DOI: 10.1177/019262330002800212
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