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Possible Involvement of bcl-2 Suppression in Wild-Type p53 Gene-Dependent Cell Growth Repression in Rat Osteosarcoma Cells

Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan

Toshifumi Tsujiuchi

Department of Oncological Pathology, Nara Medical University, Kashihara, Nara, Japan

Masahiro Tsutsumi

Department of Oncological Pathology, Nara Medical University, Kashihara, Nara, Japan

Akira Kido

Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan

Toru Morishita

Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan

Masatoshi Yoshimoto

Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan

Yoshizumi Miyauchi

Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan

Yoshio Mii

Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan

Susumu Tamai

Department of Orthopedic Surgery, Nara Medical University, Kashihara, Nara, Japan

Yoichi Konishi

Department of Oncological Pathology, Nara Medical University, Kashihara, Nara, Japan

We recently obtained 3 cloned cell lines demonstrating the p53 mutation from a lung metastatic nodule of a rat transplantable osteosarcoma. In this study, we applied wild-type p53 gene transfer to the rat osteosarcoma cells by lipofection to investigate the effects on cell growth, expression of genes such as waf1/p21, bcl-2, and bax, and nucleosomal DNA fragmentation due to apoptosis. Reconstitution of the p53 gene inhibits cellular growth, and this growth-suppressive effect is partly due to apoptosis involving bcl-2 gene suppression in this tumor type. This rat osteosarcoma model is similar in biologic behavior to human cases and thus is very suitable for further investigation of tumorigenesis and gene therapy for osteosarcoma.

Key Words: Osteosarcoma • rat • p53 • waf1/p21 • bcl-2 • bax • apoptosis

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Toxicologic Pathology, Vol. 28, No. 4, 575-579 (2000)
DOI: 10.1177/019262330002800411


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Honoki, K. Articles by Konishi, Y. Search for Related Content PubMed PubMed Citation Articles by Honoki, K. Articles by Konishi, Y. Pubmed/NCBI databases Medline Plus Health Information Bone Cancer Social Bookmarking                         What's this?