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Specific Genomic Alterations in Rat Renal Cell Carcinomas Induced by N-Ethyl-N-hydroxyethylnitrosamine

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, nkonishi{at}naramed-u.ac.jp

Mitsutoshi Nakamura

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521

Eiwa Ishida

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521

Yoichi Kawada

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521

Masayoshi Nishimine

Second Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521

Hisaki Nagai

Department of Molecular Biology, Institute of Gerontology, Nippon Medical School 1-396, Kosugi, Nakahara-ku, Kawasaki, 211-8533, Japan

Mitsuru Emi

Department of Molecular Biology, Institute of Gerontology, Nippon Medical School 1-396, Kosugi, Nakahara-ku, Kawasaki, 211-8533, Japan

To characterize genetic alterations occurring in renal tumorigenesis, EHEN-induced renal cell tumors were examined using restriction landmark genomic scanning (RLGS) analysis, an electrophoretic separation technique that detects gene amplifi cations and deletions. Comparison of DNAs from tumor against those from corresponding nontumorous kidney and /or EHEN-treated kidney without development of renal tumors yielded specific alterations in terms of both amplifi ed and reduced DNA spots. Two amplifi ed spots were detected only in renal cell tumors and an additional four spots were frequent in EHEN-treated kidneys. One reduced spot was common to all tumor samples, and another was frequently detected in the tumors analyzed but not in EHEN-treated kidneys. A subset of the altered spots thus appeared to be specific for EHEN-induced renal tumorigenesis.

Key Words: N-ethyl-N-hydroxyethylnitrosamine • rat • renal cell tumor • restriction landmark genomic scanning.

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Toxicologic Pathology, Vol. 29, No. 2, 232-236 (2001)
DOI: 10.1080/019262301317052503


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Konishi, N. Articles by Emi, M. Search for Related Content PubMed PubMed Citation Articles by Konishi, N. Articles by Emi, M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information Kidney Cancer Hazardous Substances DB N-NITROSODIETHYLAMINE Social Bookmarking                         What's this?