This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Suwa, T. Articles by Maronpot, R. R. Search for Related Content PubMed PubMed Citation Articles by Suwa, T. Articles by Maronpot, R. R. Social Bookmarking                         What's this?

A Retrospective Analysis of Background Lesions and Tissue Accountability for Male Accessory Sex Organs in Fischer-344 Rats

Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Abraham Nyska

Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, nyska{at}niehs.nih.gov.

John C. Peckham

Experimental Pathology Laboratories Inc., Research Triangle Park, North Carolina 27709

James R. Hailey

Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Joel F. Mahler

Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Joseph K. Haseman

Biostatistics Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Robert R. Maronpot

Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Because the paired lobes (ventral, dorsal, lateral, and anterior) of the rat prostate have not been consistently sampled in many carcinogenicity and toxicity studies, comparison among different investigations has been compromised. The lack of specifi c site identifi cation for prostatic lesions further lessens the value of incidences reported. We present here the lobe-specific incidences and degree of severity of background prostatic, seminal vesicular, and ampullary glandular lesions in 1768 control Fischer-344 rats from 35 recent National Toxicology Program 2-year carcinogenicity and toxicity studies conducted in 4 laboratories. The dorsal and lateral lobes were combined and considered the dorsolateral lobe where inflammation, epithelial degeneration, mucinous cysts, and edema were observed. Infl ammation in the dorsolateral lobes was signifi cantly associated with pituitary gland adenoma whose prolactin was suggested to play an important role in pathogenesi s of prostatic inflammation. Epithelial degeneration, epithelial hyperplasia, infl ammation, edema, and adenoma were conspicuous in the ventral lobes. Infl ammation and edema occurred in the anterior lobes (coagulating glands). Inflammation, dilatation, epithelial hyperplasia, edema, and adenoma were observed in the seminal vesicles. Infl ammation was also present in the ampullary glands. We suggest an optimal embedment and trimming method in rat prostate and seminal vesicle to ensure adequate, consistent sampling.

Key Words: Accessory male sex glands • background data • histopathology • rodents • pituitary gland adenoma • prolactin • spontaneous.

References

• Aronsson A., Dahlgren S., GatenbeckL., Stromberg L. (1988). Predictive sites of infl ammatory manifestation in the prostatic gland: An experimental study on nonbacterial prostatitis in the rat. Prostate 13: 17—24.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Aumü ller G., Enderle-Schmitt U., Seitz J., Müntzing J., Chandler JA (1987). Ultrastructure and immunohistochemistry of the lateral prostate in aged rats. Prostate 10: 245—256.[Medline] [Order article via Infotrieve]
• Aumü ller G., Sinowatz F. (1992). Normal development, growth, and aging in the male accessory sex glands. In: Pathobiology of the Aging Rat, vol 1, Mohr U, Dungworth DL, Capen CC (eds). ILSI Press, Washington DC, pp 427—442.
• Boorman GA, Elwell MR, Mitsumori K. (1990). Male accessory sex glands, penis, and scrotum. In: Pathology of Fischer Rats, Boorman GA, Eustis SL, Elwell MR, Montgomery Jr CA, MacKenzie WF (eds). Academic Press, San Diego, pp 419—428.
• Bosland MC (1987). Monographs on Pathology of Laboratory Animals, Genital System, Jones TC, Mohr U, Hunt RD (eds). Springer-Verlag, Berlin, pp 252—275.
• Bosland MC (1992). Lesions in the male accessory sex glands and penis. In: Pathobiology of the Aging Rat, vol 1, Mohr U, Dungworth DL, Capen CC (eds). ILSI Press, Washington, DC, pp 443—467.
• Bosland MC, Tuomari DL, Elwell MR, Shirai T., Ward JM, McConnell RF (1998). Proliferative lesions of the prostate and other accessory sex glands in male rats In: Guides for Toxicologic Pathology, STP/ARP/AFIP, Washington, DC, pp 1—20.
• Burek JD (1983). Age-associated pathology. In: Pathology of Aging Rats, CRC Press, Boca Raton, FL, pp 29—168.
• Carlton WW, Gries CL (1996). Adenoma and carcinoma, pars distalis, anterior pituitary gland, rat. In: Monographs onpathology oflaboratory animals, Endocrine system, 2nd ed, Jones TC, Capen CC, Mohr U (eds). Springer-Verlag, Berlin, pp 71—82.
• Chhabra RS, Huff JE, Schwetz BS, Selkirk J. (1990). An overview of prechronic and chronic toxicity/carcinogenicity experimental study designs and criteria used by the National Toxicology Program. Environ Health Perspect 86: 313—321.[Web of Science][Medline] [Order article via Infotrieve]
• Coleman GL, Barthold W., Osbaldiston GW, Foster SJ, Jonas AM (1977). Pathological changes during aging in barrier-reared Fischer 344 male rats. J Gerontol 32: 258—278.[Abstract/Free Full Text]
• Deerberg F., Rehm S. (1981). Secondary infl ammatory lesions of the urogenital organs in aging Han: WIST rats. Z Versuchstierkd 23: 73—76.[Medline] [Order article via Infotrieve]
• Depiante-Depaoli M., Pacheco-Rupil B (1985). Experimental autoimmunity to rat male accessory glands (MAG): Circulating antibodies, immunoglobulins bound to target glands, and immunoglobulins-secretin g cells. Am J Reprod Immunol Microbiol 7: 32—38.[Web of Science][Medline] [Order article via Infotrieve]
• Dinse GE, Haseman JK (1986). Logistic regression analysis of incidental-tumor data from animal carcinogenicity experiments. Fundam Appl Toxicol 6: 44—52.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Furth J., Nakane PK, Pasteels JL (1976). Tumours of the pituitary gland. In: Pathology of Tumours in Laboratory Animals, vol 1, part 2, Turusov VS (ed). IARC scientific publication No. 6, IARC, Lyon, pp 201—237.
• Gal R., Koren R., Nofech-Mozes S., Mukamel E., His Y., Zajdel L. (1996). Evaluation of mucinous metaplasia of the prostate gland by mucin histochemistry. Br. J Urol 77: 113—117.[Web of Science][Medline] [Order article via Infotrieve]
• Gatenbeck L., Aronsson A., Dahlgren S., Johansson B., Stromberg L. (1987). Stress stimuli-induced histopathological changes in the prostate: An experimental study in the rat. Prostate 11: 69—76.[Medline] [Order article via Infotrieve]
• Goodman DG, Ward JM, Squire RA, Chu KC, Linhart MS (1979). Neoplastic and nonneoplastic lesions in aging F344 rats. Toxicol Appl Pharmacol 48: 237—248.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Hayashi N., Sugimura Y., Kawamura J., Donjacour AA, Cunha GR (1991). Morphological and functional heterogeneity in the rat prostatic gland. Biol Reprod 45: 308—321.[Abstract]
• Holland JM, Lee C. (1980). Effects of pituitary grafts on testosterone stimulated growth of rat prostate. Biol Reprod 22: 351—355.[Abstract]
• Hsu SM, Raine L., Fanger H. (1981). Use of avidin-biotin-peroxidas e complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29: 577— 580.[Abstract]
• Ito N., Shirai T. (1990). Tumours of the accessory male sex organs. In: Pathology of Tumours in Laboratory Animals, 2nd ed, vol 1, Turusov VS, Mohr U (eds). IARC Scientific Publication No. 99, IARC, Lyon, pp 421— 443.
• Jesik CJ, Holland JM, Lee C. (1982). An anatomic and histologic study of the rat prostate. Prostate 3: 81—97.[Web of Science][Medline] [Order article via Infotrieve]
• Kaplan L., Lee C., Schaeffer AJ (1983). Effect of castration on experimental bacterial prostatitis in rats. Prostate 4: 625—630.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Kessler OJ, Keisari Y., Servadio C., Abramovici A. (1998). Role of chronic infl ammation in the promotion of prostatic hyperplasia in rats. J Urol 159: 1049—1053.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Lee C. (1981). Physiology of castration-induced regression inrat prostate. In: The Prostatic Cell: Structure and Function, PartA: Morphologic, Secretory, and Biochemical Aspects, Murphy GP, Sandberg AA, Karr JP (eds). Liss, New York, pp 145—159.
• Lee C. (1987). Gross dissection of three lobes of the rat prostate. Prog Clin Biol Res 239: 577—582.[Medline] [Order article via Infotrieve]
• Lee C., Holland JM (1987). Anatomy, histology, and ultrastructure (correlation with function), prostate, rat. In: Monographs on Pathology of Laboratory Animals, Genital System, Jones TC, Mohr U, Hunt RD (eds). Springer-Verlag, Berlin, pp 239—251.
• Lundgren R., Holmquist B., Hesselvik M., Mü ntzing J. (1984). Treatment of prostatitis in the rat. Prostate 5: 277—284.[Web of Science][Medline] [Order article via Infotrieve]
• Mitsumori K., Elwell MR (1988). Proliferative lesions in the male reproductive system of F344 rats and B6C3F1 mice: Incidence and classifi cation. Environ Health Perspect77: 11—21.[Web of Science][Medline] [Order article via Infotrieve]
• Mü ntzing J., Sufrin G., Murphy GP (1979). Prostatitis in the rat. Scand J Urol Nephrol 13: 17—22.[Web of Science][Medline] [Order article via Infotrieve]
• Nagy E., Berczi I. (1986). Immunomodulation by tamoxifen and pergolide. Immunopharmacology 12: 145—153.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• National Toxicology Program (NTP) (1997). Toxicology and Carcinogenesis Studies of Butyl Benzyl Phthalate (CAS No. 85-68-7) in F344/N Rats (Feed Studies), NTP technica l report No. 458, NIH Publication No. 97-3374. US Department of Health and Human Services, Public Health Service, NIH, NIEHS, Research Triangle Park, NC.
• National Toxicology Program (NTP) (1997). Toxicology and Carcinogenesis Studies of D&C Yellow No. 11 (CAS No. 8003-22-3) in F344/N Rats (Feed Studies), NTP technical report No. 463, NIH Publication No. 97-3379. US Department of Health and Human Services, Public Health Service, NIH, NIEHS, Research Triangle Park, NC.
• National Toxicology Program (NTP) (1997). Toxicology and Carcinogenesis Studies of Salicylazosulfapyridine (CAS No. 599-79-1) in F344/N Rats and B6C3F Mice (Gavage Studies), NTP technical report No. 457, NIH Publication No. 97-3373. US Department of Health and Human Services, Public Health Service, NIH, NIEHS, Research Triangle Park, NC.
• National Toxicology Program (NTP) (1997). Toxicology and Carcinogenesis Studies of t-Butylhydroquinone (CAS No. 1948-33- 0) in F344/N Rats and B6C3F Mice (Feed Studies), NTP technical report No. 459, NIH Publication No. 97-3375. US Department of Health and Human Services, Public Health Service, NIH, NIEHS, Research Triangle Park, NC.
• National Toxicology Program (NTP) (1998). Toxicology and Carcinogenesis Studies of Theophylline (CAS No. 58-55-9) in F344/N Rats and B6C3F Mice (Feed and Gavage Studies), NTP technical report No. 473, NIH Publication No. 98-3963. US Department of Health and Human Services, Public Health service, NIH, NIEHS, Research Triangle Park, NC.
• Pacheco-Rupil B., Depiante-Depaoli M., Casadio B. (1984). Experimental autoimmune damage to rat male accessory glands. II. T cell requirement in adoptive transfer of specifi c tissue damage. Am J Reprod Immunol 5: 15 —19.[Web of Science][Medline] [Order article via Infotrieve]
• Parker GA, Grabau J. (1987). Chronic prostatitis, rat In: Monographs on Pathology of Laboratory Animals, Genital System, Jones TC, Mohr U, Hunt RD (eds). Springer-Verlag, Berlin, pp 287—289.
• ReiterE., Hennuy B., Bruyninx M., Cornet A., Klug M., McNamara M., Closset J., Hennen G. (1999). Effects of pituitary hormones on the prostate. Prostate 38: 159—165.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• ReznikG., Hamlin II MH, Ward JM, Stinson SF (1981). Prostatichyperplasi a and neoplasia in aging F344 rats. Prostate 2: 261—268.[Web of Science][Medline] [Order article via Infotrieve]
• Robinette CL (1988). Sex-hormone-induce d infl ammation and fi bromuscular proliferation in the rat lateral prostate. Prostate 12: 271—286.[Web of Science][Medline] [Order article via Infotrieve]
• Sandusky GE, Van Pelt CS, Todd GC, Wightman K. (1988). An immunocytochemical study of pituitary adenoma s and focal hyperplasi a in old Sprague-Dawley and Fischer 344 rats. Toxicol Pathol 16: 376—380.[Web of Science][Medline] [Order article via Infotrieve]
• Scolnik MD, Servadio C., Abramovici A. (1994). Comparative study of experimentally induced benign and atypical hyperplasi a in the ventral prostate of different rat strains. J Androl 15: 287—297.[Abstract/Free Full Text]
• Shiraishi T., Kusano I., Watanabe M., Yatani R., Liu PI (1993). Mucous gland metaplasia of the prostate. Am J Surg Pathol 17: 618—622.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Shoda T., Mitsumori K., Imazawa T., Toyoda K., Tamura T., Takada K., Takahashi M. (1998). A spontaneous seminal vesicle adenocarcinoma in an aged F344 rat. Toxicol Pathol 26: 448—451.[Abstract/Free Full Text]
• Siegel S. (1956). Nonparametric Statistics for the Behavioral Sciences. McGraw-Hill, New York.
• Sikorski A (1982). Prostate lobes of aging rats: A morphometric light microscopic approach. Folia Morphol (Warsz) 41: 361—367.[Medline] [Order article via Infotrieve]
• Snedecor AW, Cochran WG (1980). Statistical Methods, 7th ed. Iowa State University Press, Ames, IA.
• Solleveld HA, Haseman JK, McConnell EE (1984). Natural history of body weight gain, survival, and neoplasia in the F344 rat. J Natl Cancer Inst 72: 929—940.[Web of Science][Medline] [Order article via Infotrieve]
• Stoker TE, Robinette CL, Britt BH, Laws SC, Cooper RL (1999). Prepubertal exposure to compounds that increase prolactin secretion in the male rat: Effects on the adult prostate. Biol Reprod 61: 1636—1643.[Abstract/Free Full Text]
• Stoker TE, Robinette CL, Cooper RL (1999). Perinatal exposure to estrogenic compounds and the subsequen t effects on the prostate of the adult rat: Evaluation of infl ammation in the ventral and lateral lobes. Reprod Toxicol 13: 463—472.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Tangbanluekal L., Robinette CL (1993). Prolactin mediates estradiol-induced infl ammation in the lateral prostate of Wistar rats. Endocrinology 132: 2407—2416.[Abstract/Free Full Text]

Toxicologic Pathology, Vol. 29, No. 4, 467-478 (2001)
DOI: 10.1080/01926230152500086


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				L. Dorso, F. Chanut, P. Howroyd, and R. Burnett Variability in Weight and Histological Appearance of the Prostate of Beagle Dogs Used in Toxicology Studies Toxicol Pathol, December 1, 2008; 36(7): 917 - 925. [Abstract] [Full Text] [PDF]

				T. Suwa, A. Nyska, J. K. Haseman, J. F. Mahler, and R. R. Maronpot Spontaneous Lesions in Control B6C3F1 Mice and Recommended Sectioning of Male Accessory Sex Organs Toxicol Pathol, February 1, 2002; 30(2): 228 - 234. [Abstract] [PDF]

				A. Nyska, A. Dayan, and R. R. Maronpot New Tools in Therapeutic Research--Prostatic Cancer and Models Toxicol Pathol, February 1, 2002; 30(2): 283 - 287. [PDF]

This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Suwa, T. Articles by Maronpot, R. R. Search for Related Content PubMed PubMed Citation Articles by Suwa, T. Articles by Maronpot, R. R. Social Bookmarking                         What's this?