This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Suwa, T. Articles by Maronpot, R. R. Search for Related Content PubMed PubMed Citation Articles by Suwa, T. Articles by Maronpot, R. R. Social Bookmarking                         What's this?

Spontaneous Lesions in Control B6C3F1 Mice and Recommended Sectioning of Male Accessory Sex Organs

Laboratory of Experimental Pathology

Abraham Nyska

Laboratory of Experimental Pathology, nyska{at}niehs.nih.gov

Joseph K. Haseman

Biostatistics Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709

Joel F. Mahler

Laboratory of Experimental Pathology

Robert R. Maronpot

Laboratory of Experimental Pathology

Because sampling of the paired lobes (ventral, dorsal, lateral, and anterior) of the mouse prostate has often been inconsistent, comparisons among different investigations have lacked validity. The absence of site identifi cation for prostatic lesions has made reported incidences relatively nonspecific. We present here the lobe-specifi cincidences and degree of severity of spontaneous lesions in prostate, coagulating gland (anterior prostatic lobe), seminal vesicles, and ampullary glands in 612 control B6C3F₁ mice from 12 recent National Toxicology Program 2-year carcinogenicity and toxicity studies conducted in 1 of 4 different laboratories. Lymphocytic infi ltration, infl ammation, epithelial hyperplasia, mucinous cyst, and mucinous metaplasia were observed in the dorsolateral lobes. Lymphocytic infi ltration, infl ammation, epithelial hyperplasia, and edema were present in the ventral lobes. Lymphocytic infi ltration, acinar dilatation, infl ammation, epithelial hyperplasia, and atrophy occurred in the coagulating glands. No neoplastic lesions were observed in the prostate or coagulating gland. Lymphocytic infi ltration, acinar dilatation, inflammation, atrophy, adenoma, adenocarcinoma, and a granular cell tumor were observed in the seminal vesicles. Lymphocytic infi ltration was also present in the ampullary glands. The results of our survey indicate that the amounts of glandular tissues were not present consistently in slides from the different laboratories. Landmarks for uniform tissue trimming are needed. We therefore suggest an optimal trimming and embedding method for mouse prostate and seminal vesicles to ensure adequate, consistent sampling.

Key Words: Background • pathology • prostate • seminal vesicle • rodent.

References

• Boorman GA, Elwell MR, Mitsumori K. (1990). Male accessory sex glands, penis, and scrotum. In: Pathology of Fischer Rat, Boorman GA, Eustis SL, Elwell MR, Montgomery Jr CA, MacKenzie WF (eds). Academic Press, San Diego, pp 419—428.
• Chandra M., Frith CH (1992). Spontaneous neoplasms in aged CD-1 mice. Toxicol Lett 61: 67—74.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Chhabra RS, Huff JE, Schwetz BS, Selkirk J. (1990). An overview of prechronic and chronic toxicity/carcinogenicity experimental study designs and criteria used by the National Toxicology Program. Environ Health Perspect 86: 313—321.[Web of Science][Medline] [Order article via Infotrieve]
• Cunha GR, Donjacour AA, Cooke PS, Mee S., Bigsby RM, Higgins SJ, Sugimura Y. (1987). The endocrinology and developmental biology of the prostate. Endocr Rev 8: 338—362.[Abstract/Free Full Text]
• Faccini JM, Abbott DP, Paulus GJJ (1990). Male genital tract. In: Mouse Histopathology, Elsevier, Amsterdam, pp 132—146.
• Finch CE, Girgis FG (1974). Enlarged seminal vesicles of senescent C57BL6J mice. J Gerontol 29: 134—138.[Abstract/Free Full Text]
• Gordon LR, Majka JA, Boorman GA (1996). Spontaneous nonneoplastic and neoplastic lesions and experimentally induced neoplasms of the testes and accessory sex glands. In: Pathobiology of theAging Mouse, Vol 1, Mohr U, Dungworth DL, Capen CC, Carlton WW, Sundberg JP, Ward JM (eds). ILSI Press, Washington DC, pp 421—441.
• Green JE, Greenberg NM, Ashendel CL, Barrett JC, Boone C., Getzenberg RH, Henkin J., Matusik R., Janus TJ, Scher HI (1998). Work-group 3: Transgenic and reconstitution models of prostate cancer. Prostate 36: 59—63.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Karbe E. (1987). Granular cell tumors of genital organs, mice. In: Monographs on Pathology of Laboratory Animals, Genital System, Jones TC, Mohr U, Hunt RD (eds). Springer-Verlag, Berlin, pp 282—286.
• Keetch DW, Humphrey P., Ratliff TL (1994). Development of a mouse model for nonbacterial prostatitis. J Urol 152: 247—250.[Web of Science][Medline] [Order article via Infotrieve]
• Maita K., Hirano M., Harada T., Mitsumori K., Yoshida A., Takahashi K., Nakashima N., Kitazawa T., Enomoto A., Inui K., Shirasu Y. (1988). Mortality, major cause of moribundity, and spontaneous tumors in CD-1 mice. Toxicol Pathol 16: 340—349.[Web of Science][Medline] [Order article via Infotrieve]
• Mitsumori K., Elwell MR (1988). Proliferative lesions in the male reproductive system of F344 rats and B6C3F1 mice: Incidence and classifi cation. Environ Health Perspect 77: 11—21.[Web of Science][Medline] [Order article via Infotrieve]
• Mitsumori K., Elwell MR (1994). Tumours of the male accessory sex glands. In: Pathology of Tumours in Laboratory Animals, Vol 2-Tumours of the Mouse, 2nd ed, Turusov VS, Mohr U (eds). IARC, Lyon, France, pp 431— 449.
• Rabstein LS, Peters RL, Spahn GJ (1973). Spontaneous tumors and pathologic lesions in SWR-J mice. J Natl Cancer Inst 50: 751—758.[Web of Science][Medline] [Order article via Infotrieve]
• Radovsky A., Mitsumori K., Chapin RE (1999). Male reproductive tract. In: Pathology of the Mouse, Maronpot RR (ed). Cache River Press, Vienna, IL, pp 381—407.
• Siegel S. (1956). Nonparametric Statistics for the Behavioral Sciences. McGraw Hill, New York.
• Sinowatz F., Amselgruber W., Plendl J., Neumü ller C. (1996). Normal development of the testes and male accessory sex organs. In: Pathobiology of the Aging Mouse, Vol 1, Mohr U, Dungworth DL, Capen CC, Carlton WW, Sundberg JP, Ward JM (eds). ILSI Press, Washington DC, pp 405—420.
• Snedecor AW, Cochran WG (1980). Statistical Methods, 7th ed. Iowa State University Press, Ames, IA.
• Sugimura Y., Sakurai M., Hayashi N., Yamashita A., Kawamura J. (1994). Age-related changes of the prostate gland in the senescence-accelerate d mouse. Prostate 24: 24—32.[Web of Science][Medline] [Order article via Infotrieve]
• Suwa T., Nyska A., Peckham JC, Hailey JR, Mahler JF, Haseman JK, Maronpot RR (2001). A retrospective analysis of background lesions and tissue accountability for male accessory sex organs in Fischer-344 rats. Toxicol Pathol 29: 467—478.[Abstract/Free Full Text]
• Tamano S., Hagiwara A., Shibata MA, Kurata Y., Fukushima S., Ito N. (1988). Spontaneous tumors in aging (C57BL/6N C3H/HeN)F1 (B6C3F1) mice. Toxicol Pathol 16: 321—326.[Web of Science][Medline] [Order article via Infotrieve]
• Ward JM, Goodman DG, Squire RA, Chu KC, Linhart MS (1979). Neoplastic and nonneoplastic lesions in aging (C57BL/6N C3H/HeN)F1 (B6C3F1) mice. J Natl Cancer Inst 63: 849—854.[Web of Science][Medline] [Order article via Infotrieve]
• Waymouth C., Coman DR, Ward-Bailey PF (1983). Spontaneous tumors of the prostate gland in inbred strains of mice. J Natl Cancer Inst 70: 199—208.[Web of Science][Medline] [Order article via Infotrieve]

Toxicologic Pathology, Vol. 30, No. 2, 228-234 (2002)
DOI: 10.1080/019262302753559560


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				A. W. Suttie, G. E. Dinse, A. Nyska, G. J. Moser, T. L. Goldsworthy, and R. R. Maronpot An Investigation of the Effects of Late-Onset Dietary Restriction on Prostate Cancer Development in the TRAMP Mouse Toxicol Pathol, April 1, 2005; 33(3): 386 - 397. [Abstract] [Full Text] [PDF]

				A. Suttie, A. Nyska, J. K. Haseman, G. J. Moser, T. R. Hackett, and T. L. Goldsworthy A Grading Scheme for the Assessment of Proliferative Lesions of the Mouse Prostate in the TRAMP Model Toxicol Pathol, January 1, 2003; 31(1): 31 - 38. [Abstract] [PDF]

				A. Nyska, A. Suttie, S. Bakshi, L. Lomnitski, S. Grossman, M. Bergman, V. Ben-Shaul, P. Crocket, J. K. Haseman, G. Moser, et al. Slowing Tumorigenic Progression in TRAMP Mice and Prostatic Carcinoma Cell Lines Using Natural Anti-Oxidant from Spinach, NAO--A Comparative Study of Three Anti-Oxidants Toxicol Pathol, January 1, 2003; 31(1): 39 - 51. [Abstract] [PDF]

This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Suwa, T. Articles by Maronpot, R. R. Search for Related Content PubMed PubMed Citation Articles by Suwa, T. Articles by Maronpot, R. R. Social Bookmarking                         What's this?