|
Sign In to gain access to subscriptions and/or personal tools.
|
Toxicologic Pathology, Vol. 30, No. 5,
606-610 (2002)
DOI: 10.1080/01926230290105811
Longitudinal Magnetic Resonance Imaging Quantitation of Rat Liver Regeneration After Partial Hepatectomy
Paul D. Hockings
GlaxoSmithKline, Welwyn, Herts, AL6 9AR, United Kingdom
Toby Roberts
GlaxoSmithKline, Welwyn, Herts, AL6 9AR, United Kingdom
Simon P. Campbell
GlaxoSmithKline, Welwyn, Herts, AL6 9AR, United Kingdom
David G. Reid
GlaxoSmithKline, Welwyn, Herts, AL6 9AR, United Kingdom, David_G_Reid{at}gsk.com
Robert W. Greenhill
GlaxoSmithKline, Welwyn, Herts, AL6 9AR, United Kingdom
Stephen R. Polley
GlaxoSmithKline, Welwyn, Herts, AL6 9AR, United Kingdom
Paul Nelson
GlaxoSmithKline, Harlow, Essex, CM19 5AD, United Kingdom
Timothy A. Bertram
GlaxoSmithKline, Welwyn, Herts, AL6 9AR, United Kingdom
Kerstin Kramer
GlaxoSmithKline, Welwyn, Herts, AL6 9AR, United Kingdom
This report demonstrates the advantages of using a noninvasive soft tissue imaging technique—magnetic resonance imaging (MRI)—to monitor liver regeneration after 70% partial hepatectomy in the rat in a longitudinal manner. Six animals were scanned prior to and on 6 subsequent occasions up to 9 days after surgical removal of the median and left lateral lobes. Within the observed time frame liver volumes were restored to approximately 88% of presurgery values. Final liver volumes correlated well with postmortem liver weights (R = 0.93). Regeneration is well-quantified empirically by a 4 parameter logistic equation: The rate of regeneration was maximal at 1.5 days, which coincided with the maximum increase of Mitotic Index—a measure of cell proliferation, determined in a subsequent study. Pre- and postpartial hepatectomy measurements remove two potentially confounding unknowns—the presurgery liver volume, and the amount of liver actually excised. 3D reconstructions of the liver effectively illustrate the morphological changes associated with the procedure, and the regrowth of liver tissue.
Key Words: MRI hepatectomy rat liver regeneration proliferation mitotic index.
References
- Arrive L., Hricak H., Goldberg HI, Thoeni RF, Margulis AR (1989). MR appearance of the liver after partial hepatectomy. AJR Am J Roentgenol 152: 1215—1220.[Abstract/Free Full Text]
- Cockman MD, Hayes DA, Kuzmak BR (1993). Motion suppression improves quantification of rat liver volume in vivo by magnetic resonance imaging. Magn Reson Med 30: 355—360.[ISI][Medline]
[Order article via Infotrieve]
- Diehl AM, Rai RM (1996). Liver regeneration 3: Regulation of signal transduction during liver regeneration. FASEB J 10: 215—227.[Abstract]
- Fan YD, Vanzieleghem B., Achten E., De Deene Y., Defreyne L., Praet M., Van Huysse J., Kunnen M., de Hemptinne B. (2001). T1 relaxation times for viability evaluation of the engrafted and the native liver in a rat model of heterotopic auxiliary liver transplantation: A pilot study. NMR Biomed 14: 350—359.[CrossRef][ISI][Medline]
[Order article via Infotrieve]
- Greco A., Stipa F., Huguet C., Gavelli A., Chieco PA, McNamara MT (1993). Early MR follow-up of partial hepatectomy. J Comput Assist Tomogr 17: 277—282.[ISI][Medline]
[Order article via Infotrieve]
- Haber BA, Mohn KL, Diamond RH, Taub R. ( 1993). Induction patterns of 70 genes during nine days after hepatectomy define the temporal course of liver regeneration. J Clin Invest 91: 1319—1326.[ISI][Medline]
[Order article via Infotrieve]
- Hashimoto M., Kothary PC, Raper SE (1999). Phenobarbital in comparison with carbon tetrachloride and phenobarbital-induce d cirrhosis in rat liver regeneration. J Surg Res 81: 164—169.[CrossRef][ISI][Medline]
[Order article via Infotrieve]
- Hashimoto M., Sanjo K. (1997). Functional capacity of the liver after two-thirds partial hepatectomy in the rat. Surgery 121: 690—697.[CrossRef][ISI][Medline]
[Order article via Infotrieve]
- Higgins RM, Anderson RM (1931). Experimental pathology of the liver. Arch Path 12: 186—202.[ISI]
- Knasmuller S., Parzefall W., Helma C., Kassie F., Ecker S., Schulte-Hermann R. (1997 ). Toxic effects of griseofulvin: Disease models, mechanisms, and risk assessment. Crit Rev Toxicol 27: 495—537.[ISI][Medline]
[Order article via Infotrieve]
- Labrecque D. (1994). Liver regeneration: A picture emerges from the puzzle. Am J Gastroenterol 89: S86—S96.[ISI][Medline]
[Order article via Infotrieve]
- Lai HS, Chung YC, Chen WJ, Chen KM ( 1992). Rat liver regeneration after partial hepatectomy: Effects of insulin, glucagon and epidermal growth factor. J Formos Med Assoc 91: 685—690.[Medline]
[Order article via Infotrieve]
- Michalopoulos GK, DeFrances MC (1997). Liver regeneration. Science 276: 60—66.[Abstract/Free Full Text]
- Murray AB, Strecker W., Silz S. ( 1980). Changes in hepatocyte volume and liver weight in rats in the early phase after partial hepatectomy. Hepatogastroenterology 27: 4—8.[Medline]
[Order article via Infotrieve]
- Ou YC, Conolly RB, Thomas RS, Xu Y., Andersen ME, Chubb LS, Pitot HC, Yang RS ( 2001). A clonal growth model: Time-course simulations of liver foci growth following partial hepatectomy. Cancer Res 61: 1879—1889.[Abstract/Free Full Text]
- Patrizio G., Pietroletti R., Pavone P., Foti N., Tettamanti E., Ventura T., Simi M., Passariello R. (1990). An animal model for the study of liver regeneration by magnetic resonance imaging. Radiol Med (Torino) 79: 453—457.[Medline]
[Order article via Infotrieve]
- Pleskovic A., Demsar F., Suput D. (1996). Assessment of liver regeneration by quantitative MRI analysis. Pflugers Arch 431: R307—R308.[CrossRef][Medline]
[Order article via Infotrieve]
- Qin YF, Van Cauteren M., Osteaux M., Willems G. (1990). Determination of liver volume in vivo in rats using MRI. Eur J Radiol 11: 191—195.[CrossRef][ISI][Medline]
[Order article via Infotrieve]
- Shamberger RC, Leichtner AM, Jonas MM, LaQuaglia MP (1996). Long-term hepatic regeneration and function in infants and children following liver resection. J Am Coll Surg 182: 515—519.[ISI][Medline]
[Order article via Infotrieve]
- Shoda T., Mitsumori K., Onodera H., Toyoda K., Uneyama C., Takada K., Hirose M. (2000). Liver tumor-promoting effect of beta-naphthoflavone, a strong CYP 1A1/2 inducer, and the relationship between CYP 1A1/2 induction and Cx32 decrease in its hepatocarcinogenesi s in the rat. Toxicol Pathol 28: 540—547.[Abstract/Free Full Text]
- Taub R. (1996). Liver regeneration in health and disease. Clin Lab Med 16: 341—360.[ISI][Medline]
[Order article via Infotrieve]
- Wilson SJ, Brereton IM, Hockings PD, Roffmann W., Doddrell DM (1993). Respiratory triggered imaging with an optical displacement sensor. Magn Reson Imaging 11: 1027—1032.[CrossRef][ISI][Medline]
[Order article via Infotrieve]
- Wood GA, Sarma DS, Archer MC (1999). Resistance to the promotion of glutathione S-transferase 7-7-positive liver lesions in Copenhagen rats. Carcinogenesis 20: 1169—1175.[Abstract/Free Full Text]

CiteULike Connotea Del.icio.us Digg Reddit Technorati What's this?
|