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Suggested Standard Operating Procedures (SOPs) for the Preparation of Electron Microscopy Samples for Toxicology/Pathology Studies in a GLP Environment

Microbiology, Pathology and Parasitology Department, College of Veterinary Medicine, Raleigh, North Carolina 27606, USA, Michael_Dykstra{at}ncsu.edu

Peter C. Mann

Experimental Pathology Laboratories, Inc., Research Triangle Park, North Carolina 27709, USA

Michael R. Elwell

Pfizer Global Research and Development, Groton, Connecticut 06340, USA

Shelley V. Ching

SVC Associates, Inc., Apex, North Carolina 27502, USA

We provide a set of Standard Operating Procedures (SOPs) for preparing samples for electron microscopic evaluation that allow storage of samples in the primary fixative for at least 17 years without noticeable degradation, do not compromise the ability to prepare the same samples for standard light microscopic evaluation, and provide tips for orientation of samples that may be necessary for evaluation. Guidelines for proper sample size, buffer composition, and fluid concentration s during processing are given. The impact of these procedures on specimen quality, ability to produce truly comparable samples for drug development studies, and ways to minimize time spent by technicians preparing these samples during necropsies is evaluated. Although many laboratories routinely employ most of these techniques, this compilation will facilitate the simultaneou s light and electron microscopic preparation by the pathologist of comparable specimens that can be stored long-term at 4°C in McDowell's and Trump's 4F:1G fixative (4F:1G).

Key Words: Sample preparation • TEM • SEM • SOPs.

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Toxicologic Pathology, Vol. 30, No. 6, 735-743 (2002)
DOI: 10.1080/01926230290166823


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Dykstra, M. J. Articles by Ching, S. V. Search for Related Content PubMed PubMed Citation Articles by Dykstra, M. J. Articles by Ching, S. V. Social Bookmarking                         What's this?