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A Fast Histochemical Staining Method to Identify Hyaline Droplets in the Rat Kidney

Organon, Department of Toxicology and Drug Disposition, 5340 BH Oss, The Netherlands, eveline.derijk{at}organon.com

Wilma T.M. Ravesloot

Organon, Department of Toxicology and Drug Disposition, 5340 BH Oss, The Netherlands

Yvonne Wijnands

Organon, Department of Toxicology and Drug Disposition, 5340 BH Oss, The Netherlands

Eric Van Esch

Organon, Department of Toxicology and Drug Disposition, 5340 BH Oss, The Netherlands

Hyaline droplet formation in the proximal tubular cells of the kidney commonly occurs under different pathological conditions in experimental animals. In rats, intracytoplasmic hyaline droplet formation is predominantly associated with accumulation of the male rat-specific 2u-globulin, whereas under other (pathological) conditions (eg, histiocytic sarcoma and chronic progressive nephropathy [CPN]) other proteins are involved. Staining methods to visualize hyaline droplets either need plastic embedded material or time-consuming (immuno)histochemical methods. A fast Chromotrope-Aniline-Blue-staining on formalin-fixed paraffin-embedded kidneys taking only 30 minutes is described. Using this method, hyaline droplets consisting of different types of proteins are easily recognized by their bright-red color.

Key Words: Kidney • hyaline droplet • Chromotrope-Aniline Blue-staining • rat 2u-globulin • nephropathy.

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Toxicologic Pathology, Vol. 31, No. 4, 462-464 (2003)
DOI: 10.1080/01926230390213775


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by De Rijk, E. P.C.T. Articles by Van Esch, E. Search for Related Content PubMed PubMed Citation Articles by De Rijk, E. P.C.T. Articles by Van Esch, E. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Social Bookmarking                         What's this?