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Hexachlorobenzene-Induced Incisor Degeneration in Sprague—Dawley Rats

Pathology Associates, A Division of Charles River Laboratories, Inc, West Chester, Ohio 45069, USA, plong2{at}cinci.rr.com

Ronald A. Herbert

National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA

Abraham Nyska

National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA

Hexachlorobenzene (HCB) was administered to female Sprague—Dawley rats in a 13-week toxicity study conducted for the National Toxicology Program (NTP). Groups of 10 rats were treated by gavage with 0.0, 0.03, 0.1, 0.3, 1.0, 3.0, 10, or 25 mg/kg HCB in corn oil 5 days/week for 13 weeks. Dose-dependent, region-specific, maxillary incisor degeneration was observed in animals given HCB at doses of 1.0 mg/kg/day and higher. Treatment with 1.0 mg/kg/day resulted in focal mesenchymal cell vacuolation and focal osteodentin formation within the pulp. Lesions were restricted to a short linear region subjacent to the odontoblast layer along the lateral aspect of the incisor at the level of the dentin-enamel interface. When observed in both incisors, lesions were bilaterally symmetric. Treatment with 3.0 mg/kg/day resulted in lateral and medial pulp lesions and focal odontoblast degeneration with dentin niche formation along the lateral incisor margin in some animals. Treatment with 10 mg/kg/day resulted in a higher incidence of lateral dentin niches and a low incidence of medial dentin niches. In animals treated with 25 mg/kg/day, a high incidence of bilaterally symmetric, lateral and medial, dentin niches were observed. The morphology and distribution of HCB-induced incisor lesions in rats are consistent with injury to a selective population of preodontoblasts.

Key Words: Rat • hexachlorobenzene • incisor • pulp • odontoblast • dentin • degeneration.

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Toxicologic Pathology, Vol. 32, No. 1, 35-40 (2004)
DOI: 10.1080/01926230490260871


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