This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Mccullough, B. Articles by Bonnefoi, M. Search for Related Content PubMed PubMed Citation Articles by Mccullough, B. Articles by Bonnefoi, M. Social Bookmarking                         What's this?

Digital Microscopy Imaging and New Approaches in Toxicologic Pathology

Aventis, Drug Safety Evaluation, Bridgewater, New Jersey 08807, USA, Bruce.McCullough{at}aventis.com

Xiaoyou Ying

Aventis, Drug Safety Evaluation, Bridgewater, New Jersey 08807, USA

Thomas Monticello

Aventis, Drug Safety Evaluation, Bridgewater, New Jersey 08807, USA

Marc Bonnefoi

Aventis, Drug Safety Evaluation, Bridgewater, New Jersey 08807, USA

Digital microscopy, a comprehensive integration of digital imaging and light microscopy, can assist the pathologist to observe, acquire, record, share, analyze, and manage pathology image data. To lead the activity for establishing new generation digital microscopy capacity, novel concepts and strategies of digital pathology information flow and digital pathology platform were designed to integrate personal digital pathology microscopy workstations and other pathology imaging modalities with centralized data storage/management. In addition, a strategy for Web-enabled interactive telepathology that would permit global capacity was designed. A novel concept of high content pathology was also created to develop an automated tissue microscopy imaging and screening approach. These new concepts, strategies, and approaches guided the development and implementation of a digital pathology platform, a telepathology platform, and automated tissue slide imaging capacity. Digital microscopy photography is now able to replace photographic film in toxicologic pathology. Digital pathology and telepathology platforms can provide a networked environment for multisite, global team participation. Our practice also ascertained the central value of digital microscopy which can provide innovative quantitative pathology information and data mining capability with various imaging biomarkers via advanced digital image processing and pathology informatics; these are now the focus of ongoing development.

Key Words: Digital microscopy • telepathology • automated tissue imaging • tissue image analysis • imaging biomarker • high content pathology • quantitative histopathology.

References

• Camp, R.L., Chung, G.G., and Rimm, D.L. (2002). Automated subcellular localization and quantification of protein expression in tissue microarrays. Nat Med 8, 1323—7.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Cross, S., Dennis, T., and Start, R. (2002). Telepathology: current status and future prospects in diagnostic histopathology. Histopathology 41(2), 91— 109.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Delnomdedieu, M., Hedlund, L., Johnson, G., and Maronpot, R. (1996). Magnetic resonance microscopy—A new tool for the toxicologic pathologist. Toxicol Pathol 24, 36—44.[Abstract/Free Full Text]
• Gareau, P.J., Wymore, A.C., Cofer, G.P., and Johnson, G.A. (2002). Imaging inflammation: direct visualization of perivascular cuffing in EAE by magnetic resonance microscopy. J Mag Res Imag 16, 28—36.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Giuliano, K.A., and Taylor, D.L. (1998). Fluorescent-protein biosensors: new tools for drug discovery. Trends Biotech 16(3), 135—40.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Gonzalez, R.C., and Woods, R.E. (2002). Digital Image Processing (2nd ed.). Addison-Wesley, Boston, MA.
• Hargreaves, A. (2003). Cell-Based Screening—An Integrated Approach to Drug Discovery. Drug Discovery Technology, Boston.
• Inoue, S. (1986). Video Microscopy, Plenum Press, New York.
• Jason, R., et al. (2003). Informatics and quantitative analysis in biological imaging. Science Special Issue: Biological imaging. Science 300, 100—2.[Abstract/Free Full Text]
• Jenkins, S. (2003). Quantitative image analysis gives more power to the pathologist. Scientist 17(6), 36.
• Kaplan, K.J., Burgess, J.R., Sandberg, G.D., et al. (2002). Use of robotic telepathology for frozen-Section diagnosis: a retrospective trial of a telepathology system for intraoperative consultation. Mod Pathol 15(11), 1197—204.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Kayser, K., Szymas, J., and Weinstein, R.S. (1999). Telepathology: Telecommunication, Electronic Education and Publication in Pathology. Springer Verlag, New York, NY.
• Kriete, A., Anderson, M.K., Love, B., Freund, J., Caffrey, J.J., Young, M.B., Sendera, T.J., Magnuson, S.R., and Braughler, J.M. (2003). Combined histomorphometric and gene-expression profiling applied to toxicology. Genome Biol 4(5), R32.[CrossRef][Medline] [Order article via Infotrieve]
• Laxman, B., Hall, D.E., Bhojani, M.S., Hamstra, D.A., Chenevert, T.L., Ross, B.D., and Rehemtulla, A. (2002). Noninvasive real-time imaging of apoptosis. Proc Natl Acad Sci USA 99(26), 16551—5.[Abstract/Free Full Text]
• Mairinger, T., Netzer, T.T., Schoner, W., and Gschwendtner, A. (1998). Pathologists' attitudes to implementing telepathology. J Telemed Telecare 4(1), 41—6 (the EUROPath project report).[Abstract/Free Full Text]
• Miano, J. (1999). Compressed Image File Formats: JPEG, PNG, GIF, XBM, BMP. Addison-Wesley, New York.
• Pickering, L. (2003). Microscopes focus on an image of developing drugs. Drug Discov Dev 6(6), 67—70.
• Purtle, H. R. (ed.) (1974). The Billings Microscope Collection of the Medical Museum Armed Forces Institute of Pathology. 2nd ed., A.F.I.P., Washington, DC.
• Rudin, M., and Weissleder, R. (2003). Molecular imaging in drug discovery and development. Nat Rev Drug Discov 2, 123—31.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Shotton, D. (ed.) (1993). Electronic Light Microscopy. John Wiley and Sons, New York.
• Shotton, D.M. (2000). From image to knowledge: the state of the art in image bioinformatics. Microsc Anal 15—17, 11.
• Sinclair, B. (2001). A sharper image: automated image analysis systems enable better cancer detection. Scientist 15(9), 23.
• Siragusa, M., and McDonnell, D.J. (2002). Indirect digital images: limit of image compression for diagnosis in endodontics. Inter Endo J 35(12), 991—5.[CrossRef]
• Smith, S.W. (2002). Digital Signal Processing: A Practical Guide for Engineers and Scientists. Newnes ( Elsevier), Burlington, MA.
• Stella, M., et al. (2003). The future looks bright. Science Special Issue: Biological imaging. Science 300, 75.[Abstract]
• Taubman, D.S., and Marcellin, M.W. (2001). JPEG2000: Image Compression Fundamentals, Standards, and Practice. Kluwer Academic Publishers, New York.
• Taylor, D.L., DeBiasio, R., LaRocca, G., Pane, D., Post, P., Kolega, J., Giuliano, K., Burton, K., Gough, B., Dow, A., et al. (1994). Potential of machine-vision light microscopy in toxicologic pathology. Toxicol Pathol 22(2), 145—59.[Abstract/Free Full Text]
• William, H.C. (2003). The Scope and History of Pathology http://www.usc. edu/hsc/dental/PTHL312abc/312a/01/01-Reader.pdf, The University of Southern California School of Dentistry, Los Angeles, CA.
• Wootton, R., Springall, D. R., Polak, J. M. (eds.) (1995). Image Analysis in Histology—Conventional and Confocal Microscopy. Cambridge University Press, London.
• Ying, X. (1995). Digital Imaging Based Measurements and Analyses in Quantitative Biomedical Research at Cellular and Microvascular Levels. Acta Univ. Oul., Oulu, Finland.
• Ying, X., Cavallo, J.S., and McCullough, B. (2001). Digital microscopy imaging in drug discovery and development. Microsc Microanal 7(S2), 622—3.
• Ying, X., and Cheung, L. (1999). Image file formats for digital microscopy. Microsc Anal 34, 9—11.
• Ying, X., and Monticello, T.M. (2002). Design of a digital microscopy imaging platform (DMIP) for pathology studies in pharmaceutical research. Microsc Microanal 8(S2), 738—9.

Toxicologic Pathology, Vol. 32, No. 2 suppl, 49-58 (2004)
DOI: 10.1080/01926230490451734


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				X. Ying and T. M. Monticello Modern Imaging Technologies in Toxicologic Pathology: An Overview Toxicol Pathol, December 1, 2006; 34(7): 815 - 826. [Abstract] [Full Text] [PDF]

This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Mccullough, B. Articles by Bonnefoi, M. Search for Related Content PubMed PubMed Citation Articles by Mccullough, B. Articles by Bonnefoi, M. Social Bookmarking                         What's this?