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Characterization of Bronchiolar Metaplasia of the Alveolar Epithelium in Female Sprague—Dawley Rats Exposed to 3,3',4,4',5-Pentachlorobiphenyl (PCB126)

Experimental Pathology Laboratories (EPL), Inc., Research Triangle Park, North Carolina 27709, USA

Michael P. Jokinen

Pathology Associates, Inc., A Charles River Company, 4915D Prospectus Drive, Durham, North Carolina 27713, USA

Nigel J. Walker

Environmental Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA

Donald M. Sells

Battelle Columbus, Columbus, Ohio 43201, USA

Abraham Nyska

Environmental Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA, nyska{at}niehs.nih.gov

To test the dioxin toxic equivalency factor methodology, the National Toxicology Program conducted a series of 2-year rat bioassays of dioxin-like compounds. Following gavage exposure of female Harlan Sprague—Dawley rats to 2,3',4,4',5-pentachlorobiphenyl (PCB126), pulmonary alveolar epithelium at the junction of terminal bronchioles and along alveolar ducts was replaced by cuboidal to columnar ciliated cells. Scattered among these were cells exhibiting characteristics consistent with those of Clara cells; they lacked cilia and had a smooth apical surface that protruded into the alveolar space. This lesion was not typical of alveolar epithelial hyperplasia seen in rodent lungs; therefore, studies were done to characterize the lesion. Results of periodic acid-Schiff (PAS) staining, alcian blue (AB) staining, and GSTPi immunohistochemical staining of the lesions seen in treated rats were more similar to normal bronchiolar epithelium than normal alveolar epithelium or alveolar epithelial hyperplasia. These findings, along with the morphology of the cells, provide evidence that this lesion is closer in character to bronchiolar epithelium than alveolar type I or alveolar type II epithelium, and as a result, was called bronchiolar metaplasia.

Key Words: PCB126 • bronchiolar metaplasia • alveolar epithelium • Toxic Equivalency Factor.

References

• Birnbaum, L.S., and DeVito, M.J. (1995). Use of toxic equivalency factors for risk assessment for dioxins and related compounds. Toxicology 105, 391—401.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Boorman, G.A., Haseman, J.K., Waters, M.D., Hardisty, J.F., and Sills, R.C. (2002). Quality review procedures necessary for rodent pathology databases and toxicogenomic studies: The National Toxicology Program experience. Toxicol Pathol 30, 88—92.[Abstract/Free Full Text]
• Burger, G.T., Renne, R.A., Sagartz, J.W., Ayres, P.H., Coggins, C.R., Mosberg, A., T., and Hayes, A.W. (1989). Histologic changes in the respiratory tract induced by inhalation of xenobiotics: Physiologic adaptation or toxicity? Toxicol Appl Pharmacol 101, 521-42.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Cheville, N.F. (1994). Cytopathology of toxic disease. In Ultrastructural Pathology An Introduction to Interpretation, 1st ed., pp. 791—877. Iowa State University Press, Ames, IA.
• Devereux, T.R. (1984). Alveolar type II and Clara cells: Isolation and xenobiotic metabolism. Environ Health Perspect 56, 95—101.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Eaton, D.L., and Bammler, T.K. (1999). Concise review of the glutathione S-transferases and their significance to toxicology. Toxicol Sci 49, 156—64.[Free Full Text]
• Hassoun, E.A., Wang, H., Abushaban, A., and Stohs, S.J. (2002). Induction of oxidative stress in the tissues of rats after chronic exposure to TCDD, 2,3,4,7,8-pentachlorodibenzofuran, and 3,3',4,4',5-pentachlorobiphenyl. J Toxicol Environ Health A 65, 825—42.[CrossRef][Web of Science]
• Kociba, R.J., Keyes, D.G., Beyer, J.E., Carreon, R.M., Wade, C.E., Dittenber, D.A., Kalnins, R.P., Frauson, L.E., Park, C.N., Barnard, S.D., Hummel, R.A., and Humiston, C.G. (1978). Results of a two-year chronic toxicity and oncogenicity study of 2,3,7,8-tetrachlorodibenzo-p-dioxin in rats. Toxicol Appl Pharmacol 46, 279—303.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Lee, M.J., and Dinsdale, D. (1994). Immunolocalization of glutathione S-transferase isoenzymes in bronchiolar epithelium of rats and mice. Am J Physiol 267(6Pt 1), L766—74.[Web of Science][Medline] [Order article via Infotrieve]
• Safe, S. (1990). Polychlorinated biphenyls (PCBs), dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and related compounds: Environmental and mechanistic considerations which support the development of toxic equivalency factors (TEFs). Crit Rev Toxicol 21, 51—88.[Web of Science][Medline] [Order article via Infotrieve]
• Tritscher, A.M., Mahler, J., Portier, C.J., Lucier, G.W., and Walker, N.J. (2000). Induction of lung lesions in female rats following chronic exposure to 2,3,7,8-tetrachlorodibenzyo-p-dioxin. Toxicol Pathol 28, 761—9.[Abstract/Free Full Text]
• Van den Berg, M., Birnbaum, L., Bosveld, A. T. C., Brunstrom, B., Cook, P., Feeley, M., Giesy, J. P., Hanberg, A., Hasegawa, R., Kennedy, S. W., Kubiak, T., Larsen, J. C., van Leeuwen, F. X., Liem, A. K., Nolt, C., Peterson, R. E., Poellinger, L., Safe, S., Schrenk, D., Tillitt, D., Tysklind, M., Younes, M., Waern, F., and Zacharewski, T. (1998). Toxic equivalency factors (TEFs) for PCBs, PCDDs, PCDFs for humans and wildlife.Environ Health Perspect 106, 775—92.[Web of Science][Medline] [Order article via Infotrieve]

Toxicologic Pathology, Vol. 32, No. 3, 333-337 (2004)
DOI: 10.1080/01926230490431817


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