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Changes in Bronchoalveolar Lavage Cells after Intratracheal Instillation of Dimethyl Selenide in Mice

ICBAS, Department of Anatomy, and UMIB, Abel Salazar Institute for Biomedical Sciences, University of Porto, Portugal, European Union, duangrud{at}yahoo.com, duangrud{at}hotmail.com, Department of Medical Science, Faculty of Science, Burapha University, Thailand

Suchart Upatham

Department of Medical Science, Faculty of Science, Burapha University, Thailand

José Carlos Oliveira

Clinical Chemistry, Department of Laboratory Pathology, Hospital of Santo António, Porto, Portugal, European Union

António Sousa Pereira

ICBAS, Department of Anatomy, and UMIB, Abel Salazar Institute for Biomedical Sciences, University of Porto, Portugal, European Union

Artur P. Águas

ICBAS, Department of Anatomy, and UMIB, Abel Salazar Institute for Biomedical Sciences, University of Porto, Portugal, European Union

CD-1 mice were exposed to a single intratracheal instillation of either 0.025 or 0.075 mg Se/kg wt of dimethyl selenide (DMSe). They were studied over 4 weeks to define the cellular inflammatory response of the airways to DMSe. Bronchoalveolar (BAL) lavage was used to collect the DMSe-induced inflammatory exudates. The DMSe instillation resulted in phlogistic responses that had the neutrophil as the main leukocyte; they were present in BAL samples, mostly at days 1 and 7. Macrophages were also increased during DMSe-induced inflammation. The lower DMSe dose resulted in an inflammatory reaction lasting for 2 weeks. Mice treated with the higher DMSe dose still showed elevated numbers of neutrophils and macrophages 4 weeks after instillation. DMSe did not change the number of lymphocytes harvested from the airways. An early increase in total protein of BAL, and late enhancement in lactate dehydrogenase was observed in mice treated with the high DMSe dose. We conclude that inhalation of DMSe triggers a moderate and dose-dependent inflammatory reaction in the mouse airways, and that this phlogistic reaction is likely to participate in the damage of respiratory epithelia that occurs upon DMSe inhalation.

Key Words: selenium • neutrophil • macrophage • inflammation • leukocyte • lymphocyte • lung.

References

• Barceloux, D.G. (1999). Selenium. J Toxicol Clin Toxicol 37(2), 145—72.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Bedwal, R.S., Nair, N., Sharma, M.P., and Mathur, R.S. (1993). Selenium—its biological perspectives. Med Hypotheses 41(2), 150—9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Bell, R.R., Soliman, M.R., Nonavinakere, V.K., Hammerbeck, D.M., andEarly, J.L. (1997a). Cadmium and/or selenium effects on guinea pig lung PGE2, TXB2 and LTC4. Res Commun Mol Pathol Pharmacol 97(2), 233—6.[Web of Science][Medline] [Order article via Infotrieve]
• Bell, R.R., Soliman, M.R., Nonavinakere, V.K., Hammerbeck, D.M., and Early, J.L. (1997b). Selenium and cadmium induced pulmonary functional impairment and cytotoxicity. Toxicol Lett 90(2—3), 107—14.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Bell, R.R., Soliman, M.R., Nonavinakere, V.K., Hammerbeck, D.M., and Early, J.L. (1998). Guinea pig pulmonary mechanics: Altered sensitivity to carbachol by cadmium and/or selenium. Lung 176(1), 15—24.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Cherdwongcharoensuk, D., Águas, A.P., Henrique, R., Upatham, S., and Pereira, A.S. (2003). Toxic effects of selenium inhalation: Acute damage of the respiratory system of mice. Hum Exp Toxicol 22, 551—7.[Abstract/Free Full Text]
• Gasmi, A., Garnier, R., Galliot-Guilly, M., Gaudillat, C., Quartenoud, B., Buisine, A., and Djebbar, D. (1997). Acute selenium poisoning. Vet Hum Toxicol 39(5), 304—8.[Web of Science][Medline] [Order article via Infotrieve]
• Gerhardsson, L., Brune, D., Nordberg, I.G.F., and Wester, P.O. (1985). Protective effect of selenium on lung cancer in smelter workers. Br J Ind. Med 42, 617—26.[Web of Science][Medline] [Order article via Infotrieve]
• Glover, J.R. (1970). Selenium and its industrial toxicology. (1970). Indust Med 39, 50—4.
• Holness, D.L., Taraschuk, G., and Nethercott, R. (1989). Health status of refinery workers its specific reference to selenium exposure. Arch Environ Health 44, 291—7.[Medline] [Order article via Infotrieve]
• Köppel, C., Baudisch, H., Beyer, K.H., Klöppel, I., and Schneider, V. (1986). Fatal poisoning with selenium dioxide. J Toxicol Clin Toxicol 24(1), 21—35.[Web of Science][Medline] [Order article via Infotrieve]
• Matoba, R., Kimura, H., Uchima, E., Abe, T., Yamada, T., Mitsukuni, Y., and Shikata, I. (1986). An autopsy case of acute selenium (selenious acid) poisoning and selenium levels in human tissues. Forensic Sci Int 31(29), 87—92.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Nantel, A.J., Brown, M., Dery, P., and Lefebvre, M. (1985). Acute poisoning by selenious acid. Vet Hum Toxicol 27(6), 531—3.[Web of Science][Medline] [Order article via Infotrieve]
• Nonavinakere, V.K., Proctor, A.S., Bell, R.R., Mallory, Z.Y., and Early, J.L. (1999). An acute intratracheal selenium study: Immediate effects on respiration in guinea pigs. Toxicol Lett 104(3), 231—7.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Oldfield, J.E. (1987). The two faces of selenium. J Nutr 117(12), 2002—8.[Abstract/Free Full Text]
• Peão, M.N.D., Águas, A.P., Sá, C.M., and Grande, N.R. (1993). The inflammatory response of the lung to tungsten particles: An experimental study in mice submitted to intratracheal instillation of a calcium tungstate powder. Lung 171(2), 187—201.[Web of Science][Medline] [Order article via Infotrieve]
• Pentel, P., Fletcher, D., and Jentzen, J. (1985). Fatal acute selenium toxicity. J Forensic Sci 30(2), 556—62.[Web of Science][Medline] [Order article via Infotrieve]
• Vinceti, M., Wei, E.T., Malagoli, C., Bergomi, M., and Vivoli, G. (2001). Adverse health effects of selenium in humans. Rev Environ Health 16(4), 233—51.[Medline] [Order article via Infotrieve]
• Wilkinson, S., and Chodak, G.W. (2003). Critical review of complementary therapies for prostate cancer. J Clin Oncol 21(11), 2199—210.[Abstract/Free Full Text]

Toxicologic Pathology, Vol. 32, No. 3, 345-350 (2004)
DOI: 10.1080/01926230490431835


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Cherdwongcharoensuk, D. Articles by Águas, A. P. Search for Related Content PubMed PubMed Citation Articles by Cherdwongcharoensuk, D. Articles by Águas, A. P. Social Bookmarking                         What's this?