This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Nyska, A. Articles by Behar, V. Search for Related Content PubMed PubMed Citation Articles by Nyska, A. Articles by Behar, V. Social Bookmarking                         What's this?

Electron Microscopy of Wet Tissues: A Case Study in Renal Pathology

Laboratory of Experimental Pathology, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, North Carolina 27709, USA, nyska{at}niehs.nih.gov

Connie A. Cummings

Pathology Associates (PAI) Division of Charles River Laboratories, Inc., Durham, North Carolina 27713, USA

Anya Vainshtein

QuantomiX, Ltd., Nes Ziona 70400, Israel

Jonathan Nadler

Harlan Biotech Israel Ltd., Kiryat Weizmann, Rehovot 76326, Israel

Nathan Ezov

Harlan Biotech Israel Ltd., Kiryat Weizmann, Rehovot 76326, Israel

Yona Grunfeld

Harlan Biotech Israel Ltd., Kiryat Weizmann, Rehovot 76326, Israel

Opher Gileadi

QuantomiX, Ltd., Nes Ziona 70400, Israel

Vered Behar

QuantomiX, Ltd., Nes Ziona 70400, Israel

In this report we introduce wet-tissue scanning electron microscopy, a novel technique for direct imaging of wet tissue samples using backscattered electrons. Samples placed in sealed capsules are imaged through a resilient, electron-transparent membrane. The contrast of the imaged samples may be enhanced by chemical staining. The samples several millimeters thick and imaged without sectioning, makes this technique suitable for rapid analysis of tissue specimens. We applied this technique to D-limonene-induced nephropathy where accumulation of hyaline protein droplets is induced in proximal and distal convoluted tubules of the kidney. Images obtained by scanning electron microscopy of hydrated kidney specimens exhibited superior resolution, contrast, and magnification compared with those obtained by conventional light microscopy of paraffin sections. The electron micrographs can be obtained within an hour of tissue removal, whereas preparation for light microscopy requires at least 1 day. These advantages of the wet scanning electron microscopy technique indicate its potential utility in a wide range of applications in histopathology and toxicology.

Key Words: Scanning electron microscopy • imaging • wet samples • kidney.

References

• Bancroft, J.D., and Gamble, M.G. (2001). Theory and Practice of Histochemical Techniques. 5th edition. Harcourt Health Sciences, New York.
• Dykstra, M.J., Mann, P.C., Elwell, M.R., Ching, S.V. (2002). Suggested standard operating procedures (SOPs) for the preparation of electron microscopy samples for toxicology/pathology studies in a GLP environment. Toxicol Pathol 30, 735—43.[Abstract/Free Full Text]
• Eddy, A.A. (1989). Interstitial nephritis induced by protein-overload proteinuria. Am J Pathol 135, 719—33.[Abstract]
• Eddy, A.A. (2001). Role of cellular infiltrates in response to proteinuria. Am J Kidney Dis 37, S25—9.[Web of Science][Medline] [Order article via Infotrieve]
• Gu, X., and Herrera, G.A. (2002). The value of electron microscopy in the diagnosis of IgA nephropathy. Ultrastruct Pathol 26, 203—10.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Gyorkey, F., Min, K.W., Krisko, I., and Gyorkey, P. (1975). The usefulness of electron microscopy in the diagnosis of human tumors. Hum Pathol 6, 421—41.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Hard, G.C. (1998). Mechanisms of chemically induced renal carcinogenesis in the laboratory rodent. Toxicol Pathol 26, 104—12.[Abstract/Free Full Text]
• Lehman-McKeeman, L.D., Rodriguez, P.A., Takigiku, R., Caudill, D., and Fey, M.L. (1989). D-Limonene induced male rat-specific nephrotoxicity: evaluation of the association between D-limonene and 2µ-globulin Toxicol Appl Pharmacol 99, 250—9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• McDowell, E.M., and Trump, B.F. (1976). Histologic fixatives suitable for diagnostic light and electron microscopy. Arch Pathol Lab Med 100, 405— 14.[Web of Science][Medline] [Order article via Infotrieve]
• National Research Council (1996). Guide for the Care and Use of Laboratory Animals. National Academy Press, Washington, DC.
• Spargo, B.H. (1975). Practical use of electron microscopy for the diagnosis of glomerular disease. Hum Pathol 6, 405—20.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Tucker, J.A. (2000). The continuing value of electron microscopy in surgical pathology. Ultrastruct Pathol 24, 383—9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]

Toxicologic Pathology, Vol. 32, No. 3, 357-363 (2004)
DOI: 10.1080/01926230490431772


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Nyska, A. Articles by Behar, V. Search for Related Content PubMed PubMed Citation Articles by Nyska, A. Articles by Behar, V. Social Bookmarking                         What's this?