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Toxicologic Pathology
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Relationships Between Organ Weight and Body/Brain Weight in the Rat: What Is the Best Analytical Endpoint?

Steven A. Bailey

Wyeth Research, Chazy, New York 12921, baileys{at}wyeth.com

Robert H. Zidell

Bristol-Myers Squibb, East Syracuse, New York 13057

Richard W. Perry

Wyeth Research, Chazy, New York 12921

Analysis of organ weight in toxicology studies is an important endpoint for identification of potentially harmful effects of chemicals. Differences in organ weight between treatment groups are often accompanied by differences in body weight between these groups, making interpretation of organ weight differences more difficult. Using data from control rats that were part of 26 toxicity studies conducted under similar conditions, we have evaluated the relationship between organ weight and body/brain weight to determine which endpoint (organ weight, organ-to-body weight ratio, or organ-to-brain weight ratio) is likely to accurately detect target organ toxicity. This evaluation has shown that analysis of organ-to-body weight ratios is predictive for evaluating liver and thyroid gland weights, and organ-to-brain weight ratios is predictive for evaluating ovary and adrenal gland weights. Brain, heart, kidney, pituitary gland, and testes weights are not modeled well by any of the choices, and alternative analysis methods such as analysis of covariance should be utilized.

Key Words: Sprague—Dawley rats • organ weight • body weight • brain weight • statistics • historical control data • toxicology studies.

References

  • Amacher, D.E., Schomaker, S.J., and Burkhardt, J.E. (1998). The relationship among microsomal enzyme induction, liver weight and histological change in rat toxicology studies. Food Chem Toxicol 36, 831—9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Angervall, L., and Carlström, E. (1963). Theoretical criteriafortheuse of relative organ weights and similar ratios in biology. J Theoret Biol 4, 254—9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Boorman, G.A., Eustis, S.L., Elwell, M.R., Montgomery, Jr., C.A., and MacKenzie, W.F. (1990). Pathology of the Fischer Rat. Academic Press, Inc., San Diego, CA.
  • Brown, D.R., Southern, L.L., and Baker, D.H. (1985). A comparison of methods for organ-weight data adjustment in chicks. Poultry Sci 64, 366—9.[Web of Science][Medline] [Order article via Infotrieve]
  • Chatamra, K., Daniel, P.M., and Lam, D.K. (1984). The effects of fasting on core temperature, blood glucose and body and organ weights in rats. Quarterly J Exp Physiol 69, 541—5.
  • Christian, J.J. (1953). Therelation of adrenal weight to body weight in mammals. Science 117, 78—80.[Free Full Text]
  • Cullen, J.W., Pare, W.P., and Mooney, A.L. (1971). Adrenal weight to body weight ratios in the Mongolian gerbil (Meriones unguiculatus). Growth 35, 169—76.[Web of Science][Medline] [Order article via Infotrieve]
  • Dikstein, S., Kaplanski, Y., Horn, G., Superstine, E., and Sulman, F.G. (1972). Drug screening for endocrine effects: influence of body weight on organ weight. J Endocrinol 54, 351—2.[Abstract/Free Full Text]
  • Dikstein, S., Kaplanski, Y., Koch, Y., Locker, D., Sulman, F.G., and Guterman, Y. (1967). Comparison of a "computer standard line method" with the "organ weight/body weight evaluation" in normal and starved female rats. Growth 31, 301—9.[Web of Science][Medline] [Order article via Infotrieve]
  • Famula, T.R., Calvert, C.C., Luna, E., and Bradford, G.E. (1988). Organ and skeletal growth in mice with a major gene for rapid postweaning growth. Growth Develop Aging 52, 145—50.
  • Feron, V.J., de Groot, A.P., Spanjers, M.T., and Til, H.P. (1973). An evaluation of the criterion "organ weight" under conditions of growth retardation. Food Cosmet Toxicol 11, 85—94.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Hart, J.E. (1990). Pituitary-related weight changes affecting the liver, uterus and adrenal glands of rats treated with hexoestrol and clomiphene in high doses. Toxicology 61, 185—94.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Hutson, J.M., Holt, A.B., Egami, K., Niall, M., Fowler, R., and Cheek, D. (1981). Compensatory renal growth in the mouse. I. Allometric approach to the effect of age. Pediat Res 15, 1370—4.[Web of Science][Medline] [Order article via Infotrieve]
  • Iwata, H., Hagiwara, T., Katoh, M., Yamamoto, S., Yamakawa, S., Shiga, A., Hirouchi, Y., Kobayashi, K., Inoue, H., and Enomoto, M. (1993). Historical control data of organ weight and gross findings in F344/DuCrJ rats and B6C3F1 mice. Exp Anim 42, 383—96.[Web of Science]
  • Keenan, K.P., Soper, K.A., Hertzog, P.R., Gumprecht, L.A., Smith, P.F., Mattson, B.A., Ballam, G.C., and Clark, R.L. (1995). Diet, overfeeding, and moderate dietary restriction in control Sprague—Dawley rats: II. Effects on age-related proliferative and degenerative lesions. Toxicol Pathol 23, 287—302.[Abstract/Free Full Text]
  • Kihara, M., Horie, R., Lovenberg, W., and Yamori, Y. (1993). Comparative study of various genetic hypertensive rat strains: blood pressure, body weight, growth and organ weights. Heart Vessels 8, 7—15.[Medline] [Order article via Infotrieve]
  • Krames, B.B., and Van Liere, E.J. (1966). The heart weight and ventricular weights of normal adult albino rats. Anat Rec 156, 461—4.[CrossRef][Medline] [Order article via Infotrieve]
  • Oishi, S., Oishi, H., and Hiraga, K. (1979). The effect of food restriction for 4 weeks on common toxicity parameters in male rats. Toxicol Appl Pharmacol 47, 15—22.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Rothacker, D.L., Kanerva, R.L., Wyder, W.E., Alden, C.L., and Maurer, J.K. (1988). Effects of variation of necropsy time and fasting on liver weights and liver components in rats. Toxicol Pathol 16, 22—6.[Abstract/Free Full Text]
  • Salsburg, D. (1986). Statistics for Toxicologists. Marcel Dekker, New York.
  • Schärer, K. (1977). The effect of chronic underfeeding on organ weights of rats. How to interpret organ weight changes in cases of marked growth retardation in toxicity tests? Toxicology 7, 45—56.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Schweisthal, M.R., Cole, Jr. T.B., and Mercer, L.P. (1982). The ability to predict weight gain, individual organ weight, and corresponding food intake in the rat by the four-parameter model for physiological responses. Anat Record 202, 131—6.[CrossRef][Medline] [Order article via Infotrieve]
  • Setnikar, I., and Magistretti, M.J. (1965). Relationships between organ weight and body weight in the male rat. Arzneim-Forsch 15, 1042—8.[Medline] [Order article via Infotrieve]
  • Shirley, E. (1977). The analysis of organ weight data. Toxicology 8, 13—22.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Shirley, E.A. (1982). The use of background data in the analysis of covariance. Statistics Med 1, 281—91.[CrossRef]
  • Shirley, E.A., and Newnham, P. (1984). The choice between analysis of variance and analysis of covariance with special reference to the analysis of organ weights in toxicology studies. Statistics Med 3, 85—91.[CrossRef]
  • Simpson, L.O., and Spears, G.F. (1973). The relationship of organ weight, body weight and age in mice. Am J Anat 137, 209—14.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Spencer, R.P. (1968). Relationship of lung weight to body length and weight. Investigative Radiology 3, 61—4.[CrossRef][Medline] [Order article via Infotrieve]
  • Stevens, M.T. (1976). The value of relative organ weights. Toxicology 5, 311—8.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Stevens, M.T. (1977). An alternative method for the evaluation of organ weight experiments. Toxicology 7, 275—81.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Sullivan, D.J. (1985). The effect of exsanguination on organ weight of rats. Toxicol Pathol 13, 229—31.[Medline] [Order article via Infotrieve]
  • Takizawa, T. (1978). An unbiased comparison of organ weights when an inequality in body weight exists. Toxicology 9, 353—60.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Teramoto, K., Tsuji, K., Saito, T., Kuhara, T., Maejima, K., Ishihara, T., and Ishibashi, M. (1996). Changes in body weight and organ weight of Ishibashi (IS) rats with growth. Exp Animals 45, 317—23.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Trieb, G., Pappritz, G., and Lutzen, L. (1976). Allometric analysis of organ weights. I. Rats. Toxicol Appl Pharmacol 35, 531—42.[CrossRef]
  • Uemitsu, N., and Nakayoshi, H. (1984). Evaluation of liver weight changes following a single oral administration of carbon tetrachloride in rats. Toxicol Appl Pharmacol 75, 1—7.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Weil, C.S., and Gad, S.C. (1980). Applications of methods of statistical analysis to efficient repeated-dose toxicologic tests. 2. Methods for analysis of body, liver, and kidney weight data. Toxicol Appl Pharmacol 52, 214—26.

Toxicologic Pathology, Vol. 32, No. 4, 448-466 (2004)
DOI: 10.1080/01926230490465874
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