This Article Free Full Text (Free OnlineFirst PDF) All Versions of this Article: 0192623308317422v1 36/4/560    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Roberts, E. S. Articles by Dorman, D. C. Search for Related Content PubMed PubMed Citation Articles by Roberts, E. S. Articles by Dorman, D. C. Pubmed/NCBI databases GEO DataSet Compound via MeSH Substance via MeSH Hazardous Substances DB HYDROGEN SULFIDE Social Bookmarking                         What's this?

Gene Expression Changes Following Acute Hydrogen Sulfide (H₂S)-induced Nasal Respiratory Epithelial Injury

^* To whom correspondence should be addressed. E-mail: david_dorman{at}ncsu.edu.

	Abstract

Hydrogen sulfide (H₂S) is a naturally occurring gas that is also associated with several industries. The potential for widespread human inhalation exposure to this toxic gas is a public health concern. The nasal epithelium is especially susceptible to H₂S-induced pathology. Injury to and regeneration of the nasal respiratory mucosa occurred in animals with ongoing H₂S exposure, suggesting that the regenerated respiratory epithelium undergoes an adaptive response and becomes resistant to further injury. To better understand this response, ten-week-old male Sprague-Dawley rats were exposed nose-only to either air or 200 ppm H₂S for three hours per day for one day or five consecutive days. Nasal respiratory epithelial cells at the site of injury and regeneration were laser capture microdissected, and gene expression profiles were generated at three, six, and twenty-four hours after the initial three-hour exposure and at twenty-four hours after the fifth exposure using the Affymetrix Rat Genome 230 2.0 microarray. Gene ontology enrichment analysis showed that H₂S exposure altered gene expression associated with a variety of biological processes, including cell cycle regulation, protein kinase regulation, and cytoskeletal organization and biogenesis. Surprisingly, our results did not show a significant change in cytochrome oxidase gene expression or bioenergetics.

First published on May 8, 2008, doi:10.1177/0192623308317422

Toxicologic Pathology 2008;36:560.

A more recent version of this article appeared on June 1, 2008


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?